1887

Abstract

The deposition of organic monolayers containing quaternary ammonium groups has been shown by many authors to confer biocidal properties on a large variety of solid surfaces. In a search for the controlling factors, the authors have grafted quaternized poly(vinylpyridine) chains on glass surfaces by two different methods and varied the charge density within the organic layer between 10 and 10 positive charges per cm. The measurements show that this parameter has a large influence on the killing efficiency. Bacterial death occurs in less than 10 min in the quiescent state above a threshold value. The value is smaller for bacteria in the growth state. It also depends on the bacterial type. An electrostatic mechanism based on the exchange of counterions between the functionalized cationic surface and the bacterial membrane is proposed and appears consistent with the results.

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2005-05-01
2019-10-14
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References

  1. Atkins, P. W. ( 1990; ). Physical Chemistry. New York: W. H. Freeman & Company.
  2. Biesalski, M. & Rühe, J. ( 1999; ). Preparation and characterization of a polyelectrolyte monolayer covalently attached to a planar solid surface. Macromolecules 32, 2309–2316.[CrossRef]
    [Google Scholar]
  3. Brass, J. M. ( 1986; ). The cell envelope of Gram-negative bacteria: new aspects of its function in transport and chemotaxis. Curr Top Microbiol Immunol 129, 1–92.
    [Google Scholar]
  4. Cen, L., Neoh, K. G. & Kang, E. T. ( 2003; ). Surface functionalization technique for conferring antibacterial properties to polymeric and cellulosic surfaces. Langmuir 19, 10295–10303.[CrossRef]
    [Google Scholar]
  5. Chen, C. Z. & Cooper, S. L. ( 2000; ). Recent advances in antimicrobial dendrimers. Adv Materials 12, 843–846.[CrossRef]
    [Google Scholar]
  6. Chen, C. Z. & Cooper, S. L. ( 2002; ). Interactions between dendrimer biocides and bacterial membranes. Biomaterials 23, 3359–3368.[CrossRef]
    [Google Scholar]
  7. Davis, B. D., Dulbecco, R., Eisen, H. N., Ginsberg, H. S. & Wood, W. B. ( 1968; ). Staphylococci. In Microbiology, chapter 24, p. 728. New York, Evanston & London: Harper and Row.
  8. Endo, Y., Tani, T. & Kodama, M. ( 1987; ). Antimicrobial activity of tertiary amine covalently bonded to a polystyrene fiber. Appl Environ Microbiol 53, 2050–2055.
    [Google Scholar]
  9. Fidai, S., Farer, S. W. & Hancock, R. E. ( 1997; ). Interaction of cationic peptides with bacterial membranes. Methods Mol Biol 78, 187–204.
    [Google Scholar]
  10. Friedrich, C. L., Moyles, D., Beverige, T. J. & Hancock, R. E. W. ( 2000; ). Antibacterial action of structurally diverse cationic peptides on gram-positive bacteria. Antimicrob Agents Chemother 44, 2086–2092.[CrossRef]
    [Google Scholar]
  11. Gottenbos, B., van der Mei, H. C., Klatter, F., Nieuwenhuis, P. & Busscher, H. J. ( 2002; ). In vitro and in vivo antimicrobial activity of covalently coupled quaternary ammonium silane coatings on silicone rubber. Biomaterials 23, 1417–1423.[CrossRef]
    [Google Scholar]
  12. Isquith, A. J., Abbott, E. A. & Walters, P. A. ( 1972; ). Surface-bonded antimicrobial activity of an organosilicon quaternary ammonium chloride. Appl Microbiol 24, 859–863.
    [Google Scholar]
  13. Kanazawa, A., Ikeda, T. & Endo, T. ( 1993; ). Polymeric phosphonium salts as a novel class of cationic biocides. III. Immobilization of phosphonium salts by surface photografting and antibacterial activity of the surface-treated polymer films. J Polym Sci Part A Polym Chem 31, 1467–1472.[CrossRef]
    [Google Scholar]
  14. Lee, S. B., Koepsel, R. R., Morley, S. W., Matyajaszewski, K., Sun, Y. & Russell, A. J. ( 2004; ). Permanent, non-leaching antibacterial surfaces. 1. Synthesis by atom transfer radical polymerization. Biomacromolecules 5, 877–882.[CrossRef]
    [Google Scholar]
  15. Lin, J., Qiu, S., Lewis, K. & Klibanov, A. M. ( 2002; ). Bactericidal properties of flat surfaces and nanoparticules derivatized with alkylated polyethyleneimines. Biotechnol Prog 18, 1082–1096.[CrossRef]
    [Google Scholar]
  16. Lin, J., Qiu, S., Lewis, K. & Klibanov, A. M. ( 2003; ). Mechanism of bactericidal and fungicidal activities of textile covalently modified with alkylated polyethyleneimine. Biotechnol Bioeng 83, 168–172.[CrossRef]
    [Google Scholar]
  17. Nikaido, H. & Nakae, T. ( 1993; ). The outer membrane of Gram-negative bacteria. In Bacillus subtilis and Other Gram-positive Bacteria, pp. 163–250. Edited by A. Sonenshein, J. Hoch & R. Losick. Washington, DC: American Society for Microbiology.
  18. Park, S. Y., Bruinsma, R. F. & Gelbart, W. M. ( 1999; ). Spontaneous overcharging of macro-ion complexes. Europhys Lett 46, 454–460.[CrossRef]
    [Google Scholar]
  19. Pelmont, J. ( 1993; ). La culture des bactéries. In Bactéries et Environnement, chapter 11, p. 258. Grenoble: Presses Universitaires de Grenoble.
  20. Poortinga, A. T., Bos, R., Norde, W. & Busscher, H. J. ( 2002; ). Electric double layer interactions in bacterial adhesion to surfaces. Surface Sci Rep 47, 1–32.[CrossRef]
    [Google Scholar]
  21. Popa, A., Davidescu, C. M., Trif, R., Ilia, Gh., Iliescu, S. & Dehelean, Gh. ( 2003; ). Study of quaternary “onium” salts grafted on polymers: antibacterial activity of quaternary phosphonium salts grafted on “gel-type” styrene–divinylbenzene copolymers. React Funct Polym 55, 151–158.[CrossRef]
    [Google Scholar]
  22. Record, M. T., Anderson, C. F. & Lohman, T. M. ( 1978; ). Thermodynamic analysis of ion effects on the binding and conformational equilibria of proteins and nucleic acids: the roles of ion association or release, screening, and ion effects on water activity. Q Rev Biophys 11, 103–178.[CrossRef]
    [Google Scholar]
  23. Rondelez, F. & Bezou, P. ( 1999; ). Des surfaces antibactériennes et autostériles. Actual Chim 10, 4–8.
    [Google Scholar]
  24. Sauvet, G., Dupond, S., Kazmierski, K. & Chojnowski, J. ( 2000; ). Biocidal polymers active by contact. V. Synthesis of polysiloxanes with biocidal activity. J Appl Polym Sci 75, 1005–1012.[CrossRef]
    [Google Scholar]
  25. Thome, J., Holländer, A., Jaeger, W., Trick, I. & Oehr, C. ( 2003; ). Ultrathin antibacterial polyammonium coatings on polymer surfaces. Surface Coating Technol 174–175, 584–587.
    [Google Scholar]
  26. Tiller, J. C., Liao, C., Lewis, K. & Klibanov, A. M. ( 2001; ). Designing surfaces that kill bacteria on contact. Proc Natl Acad Sci U S A 98, 5981–5985.[CrossRef]
    [Google Scholar]
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