@article{mbs:/content/journal/micro/10.1099/mic.0.27520-0, author = "García-Calderón, Clara B. and García-Quintanilla, Meritxell and Casadesús, Josep and Ramos-Morales, Francisco", title = "Virulence attenuation in Salmonella enterica rcsC mutants with constitutive activation of the Rcs system", journal= "Microbiology", year = "2005", volume = "151", number = "2", pages = "579-588", doi = "https://doi.org/10.1099/mic.0.27520-0", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.27520-0", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "CI, competitive index", keywords = "Km, kanamycin", abstract = "Mutations in rcsC that result in constitutive colanic acid capsule synthesis were obtained in Salmonella enterica serovar Typhimurium. Most rcsC alleles were dominant; however, recessive rcsC alleles were also found, in agreement with the postulated double role (positive and negative) of RcsC on the activation of the RcsB/C phosphorelay system. Salmonella rcsC mutants with constitutive activation of the Rcs system are severely attenuated for virulence in BALB/c mice and their degree of attenuation correlates with the level of Rcs activation. Partial relief of attenuation by a gmm mutation indicates that capsule overproduction is one of the factors leading to avirulence in constitutively activated rcsC mutants.", }