@article{mbs:/content/journal/micro/10.1099/mic.0.27518-0, author = "Pheiffer, Carmen and Betts, Joanna C. and Flynn, Helen R. and Lukey, Pauline T. and van Helden, Paul", title = "Protein expression by a Beijing strain differs from that of another clinical isolate and Mycobacterium tuberculosis H37Rv", journal= "Microbiology", year = "2005", volume = "151", number = "4", pages = "1139-1150", doi = "https://doi.org/10.1099/mic.0.27518-0", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.27518-0", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "F23, family 23", keywords = "TB, tuberculosis", keywords = "1D, one-dimensional", keywords = "IPG, immobilized pH gradient", keywords = "F11, family 11", keywords = "ICD, isocitrate dehydrogenase", keywords = "WCL, whole-cell lysate", keywords = "CF, culture filtrate", keywords = "BCIP, 5-bromo-4-chloro-indolyl-phosphatase", keywords = "BCG, Mycobacterium bovis bacille Calmette–Guérin", keywords = "NBT, nitro blue tetrazolium", keywords = "LC/MS/MS, liquid chromatography tandem mass spectrometry", keywords = "2D, two-dimensional", abstract = "The Beijing strain family has often been associated with tuberculosis (TB) outbreaks and drug resistance worldwide. In this study the authors have compared the protein expression and antigen recognition profiles of a local Beijing strain with a less prevalent clinical isolate belonging to the family 23 strain lineage, and the laboratory strain Mycobacterium tuberculosis H37Rv. Using two-dimensional electrophoresis, liquid chromatography tandem mass spectrometry and Western blot analysis several proteins were identified as quantitatively increased or decreased in both clinical strains compared to H37Rv. Remarkably, the Beijing strain showed increased expression of α-crystallin and decreased expression of Hsp65, PstS1, and the 47 kDa protein compared to the other clinical strain and H37Rv. One- and two-dimensional Western blot analysis of antigens expressed by the three strains, using plasma from TB patients, confirmed differential antigen expression by strains and patient-to-patient variation in humoral immunity. These observed protein differences could aid the elucidation of mechanisms underlying the success of the Beijing strain family, measured by global dissemination, compared to other M. tuberculosis strains.", }