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Volume 150, Issue 12

Physical and genetic map of the DSMZ 20288 chromosome and characterization of different operons by ITS analysis Free

Abstract

The DSMZ 20288 chromosome was analysed by pulsed-field gel electrophoresis, enabling the construction of a physical and genetic map. A total of 21 recognition sites of the restriction enzymes I, I-I, I and I were mapped on the chromosome, which was found to be circular with an estimated size of 2026 kb. This is believed to constitute the first study into the genomic organization of a strain of this genus, addressing the localization of important chromosomal regions such as and . A total of 23 genetic markers were mapped, including eight operons that were precisely assigned in 37 % of the chromosome. The transcription direction of loci was determined and three different clusters were recognized regarding the presence/absence of tRNA genes in ITS regions.

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Keyword(s): CHEF, counter-clamped homogeneous electric field , ITS, internal transcribed spacer and LAB, lactic acid bacteria
© SGM
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2004-12-01
2024-04-19
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Physical and genetic map of the Weissella paramesenteroides DSMZ 20288T chromosome and characterization of different rrn operons by ITS analysis
Microbiology 150, 4075 (2004); https://doi.org/10.1099/mic.0.27472-0
/content/journal/micro/10.1099/mic.0.27472-0
/content/journal/micro/10.1099/mic.0.27472-0
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