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Volume 151, Issue 2

Concordant evolution of trichothecene 3--acetyltransferase and an rDNA species phylogeny of trichothecene-producing and non-producing fusaria and other ascomycetous fungi Free

Abstract

The cereal pathogen species complex (e.g. , previously referred to as lineage 6) produces the mycotoxin trichothecene in infected grains. The fungus has a gene for self-defence, , which is responsible for 3--acetylation of the trichothecene skeleton in the biosynthetic pathway. Recently, trichothecene non-producers and (teleomorph ) were shown to have both functional () and non-functional (pseudo-) trichothecene 3--acetyltransferase genes in their genome. To gain insight into the evolution of the trichothecene genes in species, the authors examined whether or not other (pseudo-)biosynthesis-related genes are found near . However, sequence analysis of a 12 kb region containing did not result in identification of additional trichothecene (pseudo-)genes in . In a further attempt to find other trichothecene (pseudo-)genes from the non-producer, the authors examined whether or not the non-trichothecene genes flanking the ends of the core trichothecene gene cluster (i.e. the cluster) comprise a region of synteny in species. However, it was not possible to isolate trichothecene (pseudo-)genes from (in addition to the previously identified pseudo-), because synteny was not observed for this region in and . In contrast to this unsuccessful identification of additional trichothecene (pseudo-)genes in the non-producer, a functional trichothecene 3--acetyltransferase gene could be identified in fusaria other than : and ; and in an ascomycete from a different fungal genus, . Together with the recent functional identification of , these results are suggestive of a different evolutionary origin for the trichothecene 3--acetyltransferase gene from other biosynthesis pathway genes. The phylogeny of the 3--acetyltransferase was mostly concordant with the rDNA species phylogeny of these ascomycetous fungi.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): 3-ADON, 3-acetyldeoxynivalenol , DON, deoxynivalenol and NJ, neighbour-joining
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2005-02-01
2024-04-23
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Concordant evolution of trichothecene 3-O-acetyltransferase and an rDNA species phylogeny of trichothecene-producing and non-producing fusaria and other ascomycetous fungi
Microbiology 151, 509 (2005); https://doi.org/10.1099/mic.0.27435-0
/content/journal/micro/10.1099/mic.0.27435-0
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