1887

Abstract

Manganese serves an important function in in protection against oxidative stress and this bacterium can accumulate Mn up to millimolar levels intracellularly. Although the physiological role of Mn and the uptake of this metal ion have been well documented, the only uptake system described so far for this bacterium is the Mn- and Cd-specific P-type ATPase (MntA). Recently, the genome of WCFS1 has been sequenced allowing detection of genes potentially encoding Mn transport systems, using established microbial Mn transporters as the query sequence. This genome analysis revealed that WCFS1 encodes, besides the previously described gene, an ABC transport system () and three genes encoding Nramp transporters (, and ). The expression of three (, and ) of the five transport systems was specifically derepressed or induced upon Mn limitation, supporting their role in Mn homeostasis in . However, in contrast to previous reports, expression remains below detection levels in both Northern and real-time RT-PCR analysis in both Mn excess and starvation conditions. Growth of WCFS1 derivatives mutated in , or , or both and appears unaffected under Mn excess or Mn limitation. Moreover, intracellular Mn concentrations remained unaltered in these mutants compared to the wild-type. This may suggest that this species is highly adaptive in response to inactivation of these genes or, alternatively, that other transporters that have not yet been identified as Mn transporters in bacteria are involved in Mn homeostasis in .

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2005-04-01
2020-04-10
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