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Abstract

Using an established spotted cDNA microarray platform, the nature of changes in the transcriptional profiles of 2200 unique genes from the chestnut blight fungus in response to the absence of either the G subunit CPG-1 or the G subunit CPGB-1 has been explored. It is reported that 216 transcripts were altered in accumulation in the Δ-1 strain and 163 in the Δ- strain, with a considerable overlap (100 genes) that were changed in both cases. Of note, these commonly altered transcripts were changed in the same direction in every instance, thus suggesting a considerable redundancy in pathway control or extensive crosstalk. To further knowledge of the potential impact on G-protein-signalling of infection by hypovirus CHV1-EP713, the accumulation of CPG-1 and CPGB-1 was also investigated by Western analysis. It was demonstrated that both signalling components were reduced in abundance to approximately 25 % of wild-type levels, while their transcripts were slightly elevated. Comparison of a list of genes with altered expression in the presence of CHV1-EP713 to the data obtained in the absence of either G-protein subunit showed that more than one-half of all the transcripts changed by hypovirus infection were also changed in at least one G-protein mutant strain, with one-third being changed in both. Significantly, 95 % of the co-changed genes were altered in the same direction. These data provide the first evidence for modulation of G protein levels as well as the G-signalling pathways by hypovirus infection, and support the hypothesis that modification of G-protein-signalling via both G and G provides for a significant contribution to hypovirus-mediated phenotype.

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2004-12-01
2019-10-21
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Supplements

vol. , part 12, pp. 4033-4043

The following supplementary tables are available in an Acrobat PDF file.

A redundant list of clones that met the criteria for differential expression in the Δ strain when compared to EP155 by microarray.

A redundant list of clones that met the criteria for differential expression in the Δ strain when compared to EP155 by microarray.

A redundant list of clones that met the criteria for differential expression in the EP155/CHV1-EP713 strain when compared to EP155 by microarray.

In each case the AESTclone identification numbers, the -fold change together with notation denoting up or down (dn) regulation in the tested strain, the -value of the best BLASTXresult (from Dawe 2003) and the gi number and description from the NCBI database are listed.



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