1887

Abstract

Exotoxin A production in is regulated positively or negatively by several genes. Two such regulatory genes, and , which are divergently transcribed from each other, have been described previously. While computer analysis suggested that the - intergenic region contains potential binding sites for several regulatory proteins, the mechanism that regulates the expression of either or in is not known. The presence of a protein complex that specifically binds to a segment within this region was determined. In this study the binding region was localized to a 150 bp fragment of the intergenic region and the proteins that constitute the binding complex were characterized as HU and MvaT. Recombinant MvaT was purified as a fusion protein (MAL-MvaT) and shown to specifically bind to the - intergenic region. A PAO1 isogenic mutant defective in , PAOΔ, was constructed and characterized. The lysate of PAOΔ failed to bind to the 150 bp probe. The effect of on and expression was examined using real-time PCR experiments. The expression of was lower in PAOΔ than in PAO1, but no difference was detected in expression. These results suggest that MvaT positively regulates expression by binding specifically to the upstream region.

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2004-11-01
2019-10-14
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