1887

Abstract

The formation of cyclopropane fatty acid (CFA) and its role in the acid shock response in serovar Typhimurium () was investigated. Data obtained by GC/MS demonstrated that the CFA level in increased upon its entry to the stationary phase, as in other bacteria. The gene encoding CFA synthase was cloned, and mutants of the gene were constructed by allelic exchange. A mutant could not produce CFA and was sensitive to low pH. Introduction of a functional gene into a mutant cell made the mutant convert all unsaturated fatty acids to CFAs and partially restored resistance to low pH. Interestingly, the alternative sigma factor RpoS, which was induced during the stationary phase, affected the production of C CFA but not C CFA. Western blotting analysis showed that the increase in expression of CFA synthase at early stationary phase was due to the alternative sigma factor RpoS.

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2005-01-01
2019-11-18
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