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Volume 150, Issue 11

Novel organization of genes in a phthalate degradation operon of PYR-1 Free

Abstract

PYR-1 is capable of degrading polycyclic aromatic hydrocarbons (PAHs) to ring cleavage metabolites. This study identified and characterized a putative phthalate degradation operon in the PYR-1 genome. A putative regulatory protein () was encoded divergently with five tandem genes: phthalate dioxygenase large subunit (), small subunit (), phthalate dihydrodiol dehydrogenase (), phthalate dioxygenase ferredoxin subunit () and phthalate dioxygenase ferredoxin reductase (). A 6·7 kb RI fragment containing these genes was cloned into and converted phthalate to 3,4-dihydroxyphthalate. Homologues to the operon region were detected in a number of PAH-degrading spp. isolated from various geographical locations. The operon differs from those of other Gram-positive bacteria in both the placement and orientation of the regulatory gene. In addition, the PYR-1 operon contains no decarboxylase gene and none was identified within a 37 kb region containing the operon. This study is the first report of a phthalate degradation operon in spp.

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Keyword(s): PAH, polycyclic aromatic hydrocarbon , PFGE, pulsed field gel electrophoresis and TMS, trimethylchlorosilane
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2004-11-01
2023-10-02
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Novel organization of genes in a phthalate degradation operon of Mycobacterium vanbaalenii PYR-1
Microbiology 150, 3749 (2004); https://doi.org/10.1099/mic.0.27263-0
/content/journal/micro/10.1099/mic.0.27263-0
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