1887

Abstract

Application of a promoter-trapping strategy to identify plant-inducible genes carried on an indigenous plasmid, pQBR103, revealed the presence of a putative oligoribonuclease () gene that encodes a highly conserved 3′ to 5′ exoribonuclease specific for small oligoribonucleotides. The deduced amino acid sequence of the plasmid-derived ( ) showed three conserved motifs characteristic of Orn from both prokaryotes and eukaryotes. Deletion of generated no observable phenotype, but inactivation of the chromosomal copy caused slow growth in KT2440. This defect was fully restored by complementation with from ( ). Plasmid-derived was capable of partially complementing the mutant, demonstrating functionality of . Phylogenetic analysis showed that plasmid-encoded Orn was distinct from Orn encoded by the chromosome of proteobacteria. A survey of from related plasmids showed a sporadic distribution but no sequence diversity. These data suggest that the was acquired by pQBR103 in a single gene-transfer event: the donor is unknown, but is unlikely to be a member of the .

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2004-09-01
2020-09-26
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