1887

Abstract

The gene is involved in the pathway-specific positive regulation of biosynthesis of the antitumour polyketide landomycin E in 1912. LndI was overexpressed in as a protein C-terminally fused to the intein-chitin-binding-domain tag and purified in a one-step column procedure. Results of LndI titration, DNA gel mobility-shift assays and promoter-probing experiments indicate that LndI is an autoregulatory DNA-binding protein that binds to its own gene promoter and to the promoter of the structural gene . Enhanced green fluorescent protein was used as a reporter to study the temporal and spatial pattern of transcription. Expression of started before cells entered mid-exponential phase and peak expression coincided with maximal accumulation of landomycin E and biomass. In solid-phase analysis, expression was evident in substrate mycelia but was absent from aerial hyphae and spores.

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2005-01-01
2019-11-13
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