1887

Abstract

A 3·3 kb fragment from phage Ea1h in plasmid pJH94 was previously characterized and found to contain an exopolysaccharide depolymerase () gene and two additional ORFs encoding 178 and 119 amino acids. ORF178 () and ORF119 () were found to overlap by 19 bp and they resembled genes encoding lysozymes and holins. In nucleotide sequence alignments, had structurally conserved regions with residues important for lysozyme function. The gene was cloned into an expression vector and expressed in . Active lysozyme was detected only when cells with the gene and a kanamycin-resistance cassette were grown in the presence of kanamycin. Growth of was inhibited after addition of enzyme exceeding a threshold for lysozyme to target cells. When immature pears were soaked in lysates of induced cells, symptoms such as ooze formation and necrosis were retarded or inhibited after inoculation with .

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2004-08-01
2019-11-14
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