1887

Abstract

The prodigiosin biosynthesis gene cluster ( cluster) from two strains of ( ATCC 274 and sp. ATCC 39006) has been cloned, sequenced and expressed in heterologous hosts. Sequence analysis of the respective clusters revealed 14 ORFs in ATCC 274 and 15 ORFs in sp. ATCC 39006. In each species, predicted gene products showed similarity to polyketide synthases (PKSs), non-ribosomal peptide synthases (NRPSs) and the Red proteins of A3(2). Comparisons between the two clusters and the cluster from A3(2) revealed some important differences. A modified scheme for the biosynthesis of prodigiosin, based on the pathway recently suggested for the synthesis of undecylprodigiosin, is proposed. The distribution of the cluster within several sp. isolates is demonstrated and the presence of cryptic clusters in some strains shown. The cluster of ATCC 274 is flanked by and homologues and this configuration is demonstrated in several strains, whilst these genes are contiguous in strains lacking the cluster.

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2004-11-01
2019-10-14
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