1887

Abstract

The immune response and the anticandidal activity of keratinocytes and polymorphonuclear leukocytes (PMNs) play a key role in host defence against localized infection. An established model of oral candidosis based on reconstituted human oral epithelium (RHE) was supplemented with PMNs to study the effect of these immune cells during experimental oral candidosis. Infection of RHE with induced a strong expression of the chemokine interleukin-8 (IL-8) and the cytokine granulocyte-macrophages colony-stimulating factor (GM-CSF), and a moderate stimulation of interleukin-1 (IL-1), interleukin-1 (IL-1), interleukin-6 (IL-6), interferon (IFN-) and tumour necrosis factor (TNF-) by keratinocytes. This immune response was associated with chemoattraction of PMNs to the site of infection, whereas uninfected RHE failed to induce cytokine expression or to attract PMNs. Growth of the pathogen and tissue damage of -infected RHE were significantly reduced when PMNs were applied to the apical epithelial surface or when PMNs migrated through a perforated basal polycarbonate filter of the model. Notably, protection against epithelial tissue damage was also observed when PMNs were placed on the basal side of non-perforated filters, which prevented PMN migration into the RHE. Addition of PMNs enhanced a Th1-type immune response (IFN-, TNF-), down-regulated the expression of the Th2-type cytokine interleukin-10 (IL-10), and was associated with protection against -induced tissue damage. This PMN-supplemented model of oral candidosis mimics the situation, and provides a promising tool for studying the immunological interactions between keratinocytes and , as well as the influence of PMNs on pathogenesis.

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2004-09-01
2020-01-19
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