1887

Abstract

Haemolysin II, HlyII, is one of several cytotoxic proteins produced by , an opportunistic human pathogen that causes food poisoning. The gene confers haemolytic activity to cells. Here a new gene, , which is located immediately downstream of and regulates expression, is reported. The deduced amino acid sequence of HlyIIR is similar to prokaryotic DNA-binding transcriptional regulators of the TetR/AcrA family. Measurements of haemolytic activity levels and of promoter activity levels using gene fusions and primer-extension assays demonstrated that, in , transcription decreased in the presence of . Recombinant HlyIIR binds to a 22 bp inverted DNA repeat centred 48 bp upstream of the promoter transcription initiation point. transcription studies showed that HlyIIR inhibits transcription from the promoter by binding to the 22 bp repeat and RNA polymerase, and by decreasing the formation of the catalytically competent open promoter complex.

Keyword(s): RNAP, RNA polymerase
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2004-11-01
2019-10-22
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