1887

Abstract

Mu transposition complexes were used for transposon mutagenesis of strain PA68. Mu DNA transposition complexes were assembled with MuA transposase and an artificial mini-Mu transposon , and introduced into by electroporation. Eight mutants deficient in twitching motility were isolated. Southern blotting confirmed that the insertions had occurred as single events. DNA sequencing of the region flanking the insertion in the twitching-motility mutants revealed that the mini-Mu transposon had inserted into six different genes, , , , , and . Four of these have previously been proven to be needed for twitching motility, whereas the and genes have not previously been shown to be required for twitching motility. The twitching-motility defect in the mutant was partially complemented by providing the gene , and the defect in the mutant was fully complemented when was provided. A mutant and a mutant were constructed by gene replacement in the PAO1 strain. These mutants were deficient in twitching motility, showing that both the and the gene are involved in twitching motility.

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2004-08-01
2020-10-26
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