1887

Abstract

subsp. () is the aetiological agent of American foulbrood (AFB), the most virulent bacterial disease of honey bee brood worldwide. In many countries AFB is a notifiable disease since it is highly contagious, in most cases incurable and able to kill affected colonies. Genotyping of field isolates of revealed at least four genotypes (, , and ) present in Germany which are genotypically different from the reference strain DSM 7030. Therefore, based on these data, five different genotypes of are now identified with genotype standing out with a characteristic brown-orange and circled two-coloured colony morphology. Analysing the metabolic profiles of three German genotypes (, and ) as well as of the reference strain using the Biolog system, a characteristic biochemical fingerprint could be obtained for each strain. Cluster analysis showed that while genotypes , and the reference strain DSM 7030 are rather similar, genotype is clearly different from the others. Analysis of all isolates for plasmid DNA revealed two different plasmids present only in isolates belonging to genotype . Therefore, genotype is remarkable in all aspects analysed so far. Future analysis will show whether or not these differences will expand to differences in virulence.

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2004-07-01
2019-10-14
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