RT Journal Article SR Electronic(1) A1 Oura, Takahiro A1 Kajiwara, SusumuYR 2004 T1 Saccharomyces kluyveri FAD3 encodes an ω3 fatty acid desaturase JF Microbiology, VO 150 IS 6 SP 1983 OP 1990 DO https://doi.org/10.1099/mic.0.27049-0 PB Microbiology Society, SN 1465-2080, AB Fungi, like plants, are capable of producing the 18-carbon polyunsaturated fatty acids linoleic acid and α-linolenic acid. These fatty acids are synthesized by catalytic reactions of Δ12 and ω3 fatty acid desaturases. This paper describes the first cloning and functional characterization of a yeast ω3 fatty acid desaturase gene. The deduced protein encoded by the Saccharomyces kluyveri FAD3 gene (Sk-FAD3) consists of 419 amino acids, and shows 30–60 % identity with Δ12 fatty acid desaturases of several eukaryotic organisms and 29–31 % identity with ω3 fatty acid desaturases of animals and plants. During Sk-FAD3 expression in Saccharomyces cerevisiae, α-linolenic acid accumulated only when linoleic acid was added to the culture medium. The disruption of Sk-FAD3 led to the disappearance of α-linolenic acid in S. kluyveri. These findings suggest that Sk-FAD3 is the only ω3 fatty acid desaturase gene in this yeast. Furthermore, transcriptional expression of Sk-FAD3 appears to be regulated by low-temperature stress in a manner different from the other fatty acid desaturase genes in S. kluyveri., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.27049-0