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Volume 150, Issue 8

The impact of different intensities of green light on the bacteriochlorophyll homologue composition of the chlorobiaceae and Free

Abstract

Members of the and are unique among the phototrophic micro-organisms in having a remarkably rich chlorophyll pigment diversity. The physiological regulation of this diversity and its ecological implications are still enigmatic. The bacteriochlorophyll composition of the chlorobiaceae strain CE 2404 and strain UdG 6030 was therefore studied by both HPLC with photodiode array (PDA) detection and liquid chromatography-mass spectrometry (LC-MS). These strains were grown in liquid cultures under green light (480–615 nm) at different light intensities (0·2–55·7 μmol photons m s), simulating the irradiance regime at different depths of the water column of deep lakes. The specific growth rates of under green light achieved a maximum of 0·06 h at light intensities exceeding 6 μmol photons m s, lower than the maximum observed under white light (approx. 0·1 h). The maximal growth rates of under green light were slightly higher (0·07 h) than observed for and were achieved at 3·5 and 4·3 μmol photons m s. LC-MS/MS analysis of pigment extracts revealed most (>90 %) BChl homologues of to be esterified with farnesol. The homologues differed in mass by multiples of 14 Da, reflecting different alkyl subsituents at positions C-8 and C-12 on the tetrapyrrole macrocycle. The relative proportions of the individual homologues varied only slightly among different light intensities. The specific content of BChl was maximal at 3–5 μmol photons m s [400±150 nmol BChl (mg protein)]. In the case of , the specific content of BChl was maximal at 4·3 μmol photons m s [115 nmol BChl (mg protein)], and this species was characterized by high carotenoid (isorenieratene) contents. The major BChl forms were esterified with a range of isoprenoid and straight-chain alcohols. The major isoprenoid alcohols comprised mainly farnesol and to a lesser extent geranylgeraniol. The straight-chain alcohols included C, C, C, C and C. Interestingly, the proportion of straight alkyl chains over isoprenoid esterified side chains shifted markedly with increasing light intensity: the isoprenoid side chains dominated at low light intensities, while the straight-chain alkyl substituents dominated at higher light intensities. The authors propose that this phenomenon may be explained as a result of changing availability of reducing power, i.e. the highly reduced straight-chain alcohols have a higher biosynthetic demand for NADPH than the polyunsaturated isoprenoid with the same number of carbon atoms.

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Keyword(s): BChl, bacteriochlorophyll , LC-MS, liquid chromatography-mass spectrometry , LC-MS/MS, liquid chromatography-tandem mass spectrometry , PDA, photodiode array and TMC, tetrapyrrole macrocycle
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2004-08-01
2024-04-20
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The impact of different intensities of green light on the bacteriochlorophyll homologue composition of the chlorobiaceae Prosthecochloris aestuarii and Chlorobium phaeobacteroides
Microbiology 150, 2555 (2004); https://doi.org/10.1099/mic.0.27048-0
/content/journal/micro/10.1099/mic.0.27048-0
/content/journal/micro/10.1099/mic.0.27048-0
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