1887

Abstract

Under conditions of iron limitation, secretes a high-affinity siderophore pyoverdine to scavenge Fe(III) in the extracellular environment and shuttle it into the cell. Uptake of the pyoverdine–Fe(III) complex is mediated by a specific outer-membrane receptor protein, FpvA (ferripyoverdine receptor). Three siderovars can be distinguished, each producing a different pyoverdine (type I–III) and a cognate FpvA receptor. Growth of an mutant of PAO1 (type I) under iron-limiting conditions can still be stimulated by its cognate pyoverdine, suggesting the presence of an alternative uptake route for type I ferripyoverdine. analysis of the PAO1 genome revealed that the product of gene PA4168 has a high similarity with FpvA. Inactivation of PA4168 (termed ) in an mutant totally abolished the capacity to utilize type I pyoverdine. The expression of is induced by iron limitation in Casamino acids (CAA) and in M9-glucose medium, but, unlike , not in a complex deferrated medium containing glycerol as carbon source. The gene was also detected in other isolates, including strains producing type II and type III pyoverdines. Inactivation of the homologues in these strains impaired their capacity to utilize type I ferripyoverdine as a source of iron. Accordingly, introduction of restored the capacity to utilize type I ferripyoverdine.

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2004-06-01
2019-10-14
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