1887

Abstract

Protein -glycosylation is essential for protein modification and plays important roles in eukaryotic cells. -Mannosylation of proteins occurs in the filamentous fungus . The structure and function of the gene, encoding protein --mannosyltransferase, which is responsible for the initial -mannosylation reaction in , was characterized. Disruption of the gene resulted in the reduction of protein --mannosyltransferase activity to 6 % of that of the wild-type strain and led to underglycosylation of an extracellular glucoamylase. The disruptant exhibited abnormal cell morphology and alteration in carbohydrate composition, particularly reduction in the skeletal polysaccharides in the cell wall. The results indicate that PmtA is required for the formation of a normal cell wall in .

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2004-06-01
2019-12-05
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