1887

Abstract

Sensitivity to class IIa bacteriocins from lactic acid bacteria was recently associated with the mannose phosphotransferase system (PTS) permease, , in . To assess the involvement of this protein complex in class IIa bacteriocin activity, the operon, encoding , was heterologously expressed in an insensitive species, namely , using the NICE double plasmid system. Upon induction of the cloned operon, the recombinant became sensitive to leucocin A. Pediocin PA-1 and enterocin A also showed inhibitory activity against cultures expressing . Furthermore, the role of the three genes of the operon was investigated. Derivative plasmids containing various combinations of these three genes were made from the parental plasmid by divergent PCR. The results showed that expression of alone is sufficient to confer sensitivity to class IIa bacteriocins in .

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2004-08-01
2019-10-23
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