1887

Abstract

In , acetate is formed by acetaldehyde dehydrogenase (ACDH), a key enzyme of the pyruvate dehydrogenase (PDH) bypass, which fulfils the essential task of generating acetyl-CoA in the cytosol. The role of the five members of the ACDH family ( genes) was investigated during anaerobic growth on glucose. Single and multiple Δ mutants were generated in the wine-yeast-derived V5 and laboratory CEN.PK strains and analysed under standard (YPD 5 % glucose) and wine (MS 20 % glucose) fermentation conditions. The deletion of and decreased acetate formation in both strains, demonstrating for the first time that the mitochondrial Ald5p isoform is involved in the biosynthesis of acetate during anaerobic growth on glucose. Acetate production of the Δ mutant was slightly decreased in the CEN.PK strain during growth on YPD only. In contrast, the deletion of or had no effect on acetate production. The absence of Ald6p was compensated by the mitochondrial isoforms and this involves the transcriptional activation of . Consistent with this, growth retardation was observed in ΔΔ, and this effect was amplified by the additional deletion of . A Δ null mutant, devoid of ACDH activity, was viable and produced similar levels of acetate to the ΔΔΔ strain, excluding a role of Ald2p and Ald3p. Thus, acetate is mainly produced by the cytosolic PDH bypass via Ald6p and by a mitochondrial route involving Ald5p. An unknown alternative pathway can compensate for the loss of Ald6p, Ald4p and Ald5p.

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2004-07-01
2019-11-21
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