1887

Abstract

The basis of fructophily in the yeast has been shown to reside in the performance of transport systems for hexoses. In this study, a gene encoding a fructose-specific transporter was characterized. The strategy involved the functional complementation of a strain that does not take up hexoses ( strain). This strain was transformed with a genomic library of . One transformant capable of growing on fructose, but not on glucose, was obtained. This transformant did not transport -[C]glucose, and the kinetic parameters for -[C]fructose were =3·3 mmol h g and =80·4 mM. As in the original strain of , fructose uptake was not inhibited by the presence of other hexoses or uranyl. The plasmid responsible for the observed phenotype was found to carry an ORF encoding a 616 amino acid protein with the characteristics of a membrane transporter, which was designated (fructose facilitator ). The impairment in function observed in an transformant expressing a truncated Ffz1 protein lacking 67 amino acids at the C-terminus suggests an important role for this terminal part in the proper structure of the transporter.

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2004-07-01
2019-11-20
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