RT Journal Article SR Electronic(1) A1 Reimers, Jacqueline M. A1 Schmidt, Karen H. A1 Longacre, Angelika A1 Reschke, Dennis K. A1 Wright, Barbara E.YR 2004 T1 Increased transcription rates correlate with increased reversion rates in leuB and argH Escherichia coli auxotrophs JF Microbiology, VO 150 IS 5 SP 1457 OP 1466 DO https://doi.org/10.1099/mic.0.26954-0 PB Microbiology Society, SN 1465-2080, AB Escherichia coli auxotrophs of leuB and argH were examined to determine if higher rates of transcription in derepressed genes were correlated with increased reversion rates. Rates of leuB and argH mRNA synthesis were determined using half-lives and concentrations, during exponential growth and at several time points during 30 min of amino acid starvation. Changes in mRNA concentration were primarily due to increased mRNA synthesis and not to increased stability. Four strains of E. coli amino acid auxotrophs, isogenic except for relA and argR, were examined. In both the leuB and argH genes, rates of transcription and mutation were compared. In general, strains able to activate transcription with guanosine tetraphosphate (ppGpp) had higher rates of mRNA synthesis and mutation than those lacking ppGpp (relA2 mutants). argR knockout strains were constructed in relA + and relA mutant strains, and rates of both argH reversion and mRNA synthesis were significantly higher in the argR knockouts than in the regulated strains. A statistically significant linear correlation between increased rates of transcription and mutation was found for data from both genes. In general, changes in mRNA half-lives were less than threefold, whereas changes in rates of mRNA synthesis were often two orders of magnitude. The results suggest that specific starvation conditions target the biosynthetic genes for derepression and increased rates of transcription and mutation., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.26954-0