1887

Abstract

Two closely related but compatible plasmids of the IncQ-2 and IncQ-2 groups, pTF-FC2 and pTC-F14, were discovered in two acidiphilic chemolithotrophic bacteria. Cross-complementation and cross-regulation experiments by the replication proteins were carried out to discover what changes were necessary when the plasmids evolved to produce two incompatibility groups. The requirement of a pTC-F14 for a RepC DNA-binding protein was plasmid specific, whereas the requirement for the RepA helicase and RepB primase was less specific and could be complemented by the IncQ-2 plasmid pTC-FC2, and the IncQ-1 plasmid pIE1108. None of the IncQ-1 plasmid replication proteins could complement the pTC-F14 , and pTC-F14 and RSF1010 were incompatible. This incompatibility was associated with the RepC replication protein and was not due to iteron incompatibility. Replication of pTC-F14 took place from a 5·7 kb transcript that originated upstream of the gene located within the region required for mobilization. A pTC-F14 fusion was regulated by the pTC-F14 gene product and was plasmid specific, as it was not regulated by the RepB proteins of pTF-FC2 or the IncQ-1 and IncQ-1 plasmids. Plasmid pTC-F14 appears to have evolved independently functioning iterons and a plasmid-specific RepC-binding protein; it also has a major replication transcript that is independently regulated from that of pTF-FC2. However, the RepA and RepB proteins have the ability to function with either replicon.

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2004-06-01
2020-01-22
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References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K.. 1993; Current Protocols in Molecular Biology New York: Wiley Interscience;
  2. Casadaban M. J., Martinez-Arias A., Shapira S. K., Chou J.. 1983; β-Galactosidase gene fusions for analyzing gene expression inEscherichia coli and yeast. Methods Enzymol100:293–308
    [Google Scholar]
  3. Deane S. M., Rawlings D. E.. 2004; Plasmid evolution and interaction between the plasmid addiction stability systems of two related broad-host-range IncQ-like plasmids. J Bacteriol186:2123–2133[CrossRef]
    [Google Scholar]
  4. Derbyshire K. M., Hatfull G., Willetts N. S.. 1987; Mobilization of the non-conjugative plasmid RSF1010: a genetic analysis of its origin of transfer. Mol Gen Genet206:154–160[CrossRef]
    [Google Scholar]
  5. Dorrington R. A., Rawlings D. E.. 1989; Identification and sequence of the basic replication region of a broad-host-range plasmid isolated from Thiobacillus ferrooxidans. J Bacteriol171:2735–2739
    [Google Scholar]
  6. Dorrington R. A., Bardien S., Rawlings D. E.. 1991; The broad-host-range plasmid pTF-FC2 requires a primase-like protein for autonomous replication in Escherichia coli. Gene108:7–14[CrossRef]
    [Google Scholar]
  7. Frey J., Bagdasarian M.. 1989; The molecular biology of IncQ plasmids. In Promiscuous Plasmids of Gram-Negative Bacteria pp.79–93 Edited by Thomas C. M.. London: Academic Press;
  8. Gardner M. N., Deane S. M., Rawlings D. E.. 2001; Isolation of a new broad-host-range IncQ-like plasmid, pTC-F14, from the acidophilic bacterium Acidithiobacillus caldus and analysis of the plasmid replicon. J Bacteriol183:3303–3309[CrossRef]
    [Google Scholar]
  9. Haring V., Scherzinger E.. 1989; Replication proteins of IncQ plasmid RSF1010. In Promiscuous Plasmids of Gram-Negative Bacteria pp.95–124 Edited by Thomas C. M.. London: Academic Press;
  10. Harley C. B., Reynolds R. P.. 1987; Analysis of E. coli promoter sequences. Nucleic Acids Res15:2343–2361[CrossRef]
    [Google Scholar]
  11. Honda Y., Sakai H., Hiasa H., Tanaka K., Komano T., Bagdasarian M.. 1991; Functional division and reconstruction of a plasmid replication origin: molecular dissection of the oriV of the broad-host-range plasmid RSF1010. Proc Natl Acad Sci U S A88:179–183[CrossRef]
    [Google Scholar]
  12. Kim K., Meyer R. J.. 1986; Copy-number of broad host-range plasmid R1162 is regulated by a small RNA. Nucleic Acids Res14:8027–8046[CrossRef]
    [Google Scholar]
  13. Kim Y.-J., Meyer R. J.. 1991; An essential iteron-binding protein required for plasmid R1162 replication induces localized melting within the origin at a specific site in AT-rich DNA. J Bacteriol173:5539–5545
    [Google Scholar]
  14. Laing E., Pretorius I. S.. 1992; Synthesis and secretion of an Erwinia chrysanthemi pectate lyase in Saccharomyces cerevisiae regulated by different combinations of bacterial and yeast promoter and signal sequences. Gene121:35–45[CrossRef]
    [Google Scholar]
  15. Lin L.-S., Meyer R. J.. 1987; DNA synthesis is initiated at two positions within the origin of replication of plasmid R1162. Nucleic Acids Res15:8319–8331[CrossRef]
    [Google Scholar]
  16. Lin L.-S., Kim Y.-J., Meyer R. J.. 1987; The 20 bp, directly repeated DNA sequence of broad host range plasmid R1162 exerts incompatibility in vivo and inhibits R1162 DNA replication in vitro. Mol Gen Genet208:390–397[CrossRef]
    [Google Scholar]
  17. Miao D.-M., Honda Y., Tanaka K., Higashi A., Nakamura T., Taguchi Y., Sakai H., Komano T., Bagdasarian M.. 1993; A base-paired hairpin structure essential for the functional priming signal for DNA replication of the broad host range plasmid RSF1010. Nucleic Acids Res21:4900–4903[CrossRef]
    [Google Scholar]
  18. Miao D.-M., Sakai H., Okamoto S., Tanaka K., Okuda M., Honda Y., Komano T., Bagdasarian M.. 1995; The interaction of RepC initiator with iterons in the replication of the broad host-range plasmid RSF1010. Nucleic Acids Res23:3295–3300[CrossRef]
    [Google Scholar]
  19. Miller J. H.. 1972; Experiments in Molecular Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
  20. Novick R. P.. 1987; Plasmid incompatibility. Microbiol Rev51:381–395
    [Google Scholar]
  21. Persson C., Nordström K.. 1986; Control of replication of the broad host range plasmid RSF1010: the incompatibility determinant consists of directly repeated sequences. Mol Gen Genet203:189–192[CrossRef]
    [Google Scholar]
  22. Rawlings D. E., Tietze E.. 2001; Comparative biology of IncQ and IncQ-like plasmids. Microbiol Mol Biol Rev65:481–496[CrossRef]
    [Google Scholar]
  23. Rawlings D. E., Woods D. R.. 1985; Mobilization of Thiobacillus ferrooxidans plasmids among Escherichia coli strains. Appl Environ Microbiol49:1323–1325
    [Google Scholar]
  24. Rawlings D. E., Pretorius I., Woods D. R.. 1984; Expression of a Thiobacillus ferrooxidans origin of replication in Escherichia coli. J Bacteriol158:737–738
    [Google Scholar]
  25. Sambrook J., Fritsch E. F., Maniatis T.. 1989; Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
  26. Scholz P., Haring V., Wittmann-Liebold B., Ashman K., Bagdasarian M., Scherzinger E.. 1989; Complete nucleotide sequence and gene organization of the broad-host-range plasmid RSF1010. Gene75:271–288[CrossRef]
    [Google Scholar]
  27. Smalla K., Heuer H., Gotz A., Niemeyer D., Krogerrecklenfort E., Tietze E.. 2000; Exogenous isolation of antibiotic resistance plasmids from piggery manure slurries reveals a high prevalence and diversity of IncQ-like plasmids. Appl Environ Microbiol66:4854–4862[CrossRef]
    [Google Scholar]
  28. Smith A. S., Rawlings D. E.. 1997; The poison-antidote stability system of the broad-host-range Thiobacillus ferrooxidans plasmid pTF-FC2. Mol Microbiol26:961–970[CrossRef]
    [Google Scholar]
  29. Smith A. S., Rawlings D. E.. 1998; Efficiency of the pTF-FC2 pas poison-antidote stability system in Escherichia coli is affected by the host strain, and antidote degradation requires the Lon protease. J Bacteriol180:5458–5462
    [Google Scholar]
  30. Snyder L., Champness W.. 2003; Molecular Genetics of Bacteria Washington, DC: American Society for Microbiology;
    [Google Scholar]
  31. Sýkora P.. 1992; Macroevolution of plasmids: a model for plasmid speciation. J Theor Biol159:53–65[CrossRef]
    [Google Scholar]
  32. Tietze E.. 1998; Nucleotide sequence and genetic characterization of the novel IncQ-like plasmid pIE1107. Plasmid39:165–181[CrossRef]
    [Google Scholar]
  33. Yanisch-Perron C., Vieira J., Messing J.. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene33:103–119[CrossRef]
    [Google Scholar]
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