1887

Abstract

The enzyme LuxS is responsible for the production of autoinducer-2 (AI-2), a molecule that has been implicated in quorum sensing in many bacterial species. This study investigated whether there is a -dependent signalling system in the Gram-negative bacteria spp. is a broad-host-range pathogen and an important cause of nosocomial infections. Production of AI-2 activity was detected in ATCC 274 and ATCC 39006 and their genes were sequenced. mutants were constructed in these strains and were analysed to determine which phenotypes are regulated by and therefore, potentially, by AI-2. The phenotypes of the mutants included decreased carbapenem antibiotic production in ATCC 39006 and decreased prodigiosin and secreted haemolysin production in ATCC 274. The mutant of ATCC 274 was also found to exhibit modestly reduced virulence in a model. Finally, it was shown that the culture supernatant of a wild-type strain contains a signal, presumably AI-2, capable of complementing the prodigiosin defect of the mutant of another strain, even when substantially diluted. It is concluded that modulates virulence and antibiotic production in , in a strain-dependent manner, and that, for at least one phenotype, this regulation is via extracellular signalling.

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2004-06-01
2020-04-03
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