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Volume 150, Issue 5

Similar genotypes of in different forms of infection Free

Abstract

This study describes the genotyping of organisms isolated from three groups of patients that developed diverse forms of infection; the patients were monitored in the same French hospital. Forty archival specimens from 13 adults with pneumonia, eight adults colonized by and 19 immunocompetent infants infected with the fungus contemporaneously with a bronchiolitis episode were analysed retrospectively. Genotyping was performed by analysis of sequences of the internal transcribed spacer (ITS)1 and ITS2 regions, and of the dihydropteroate synthase (DHPS) locus. At the ITS regions, a high diversity of genotypes, identical main genotypes (Ba and Ba) and the occurrence of mixed infections (more than one genotype) were observed in the three patient groups. At the DHPS locus, the results indicated the presence of mutants in the two adult groups, as well as in the infant group. Consequently, at these two independent genomic regions, isolates from patients who developed different forms of infection and who lived in the same geographical region presented common characteristics. These results suggest that patients infected with , whatever the form of infection they present, are part of a common human reservoir for .

  • Received:
  • Accepted:
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  • Published Online:
Keyword(s): DHPS, dihydropteroate synthase , ITS, internal transcribed spacer and PCP, Pneumocystis pneumonia
SGM
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2004-05-01
2024-04-18
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Similar genotypes of Pneumocystis jirovecii in different forms of Pneumocystis infection
Microbiology 150, 1173 (2004); https://doi.org/10.1099/mic.0.26919-0
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