1887

Abstract

Pathogenesis of is controlled to a major extent by the two quorum-sensing systems and . The previously uncharacterized gene was identified as a major virulence regulator, , in the quorum-sensing hierarchy. is a member of the LuxR family and possesses a box in its upstream region. Transposon inactivation of abrogated the production of -acylhomoserine lactones and the secretion of exoproducts and diminished bacterial virulence for . Cytotoxicity towards macrophages was not affected. mRNA was expressed more strongly in the presence of human serum and oxidative stress than under standard growth conditions. High-density oligonucleotide microarrays were used to compare the global expression profile of a wild-type strain and a mutant. One-hundred-and-fifty-one and 113 genes were significantly differentially expressed in the presence of HO and human serum, respectively. The disruption of repressed the expression of genes that are known to be promoted by quorum sensing and activated the expression of genes that are known to be repressed by quorum sensing. Moreover, the mutant harboured less mRNA transcript for the production of siderophores and membrane-bound elements of antibiotic resistance. The protein encoded by regulates several traits of pathogenicity; hence, the name (‘virulence and quorum-sensing regulator’) was assigned to .

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2004-04-01
2019-10-15
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