1887

Abstract

The genotype of isolated from humans and animals is highly polymorphic. Therefore, it is important to compare the genotypes of isolates from humans and animals to determine the zoonotic potential of animal isolates. PCR-based genotype classification using known sequence-tagged site (STS) primers allows identification of zoonotic isolates of animal origin. To this end, 51 isolates from monkeys, cattle, pigs, chickens, quails and pheasants were subjected to genotype analysis using seven kinds of STS primers. Out of the 51 isolates, 39 were identified as one of the known genotypes, four showed mixed genotypes, and eight were unknown genotypes as these were negative for all STS primers. When these results were combined with previous studies on 41 isolates from animals and compared with the diversity of genotypes of 102 human isolates, 67·4 % (62/92) of isolates from mammals and birds were identical to human genotypes. Since the unknown genotype of human origin had been placed into an additional clade in the small-subunit rRNA gene phylogeny, further molecular study on the eight isolates of unknown genotype from the present study will facilitate our understanding of their zoonotic potential.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.26899-0
2004-05-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/micro/150/5/mic1501147.html?itemId=/content/journal/micro/10.1099/mic.0.26899-0&mimeType=html&fmt=ahah

References

  1. Abe, N., Nagoshi, M., Takami, K., Sawano, Y. & Yoshikawa, H. ( 2002; ). A survey of Blastocystis sp. in livestock, pets, and zoo animals in Japan. Vet Parasitol 106, 203–212.[CrossRef]
    [Google Scholar]
  2. Abe, N., Wu, Z. & Yoshikawa, H. ( 2003a; ). Molecular characterization of Blastocystis isolates from birds by PCR with diagnostic primers and restriction fragment length polymorphism analysis of the small subunit ribosomal RNA gene. Parasitol Res 89, 393–396.
    [Google Scholar]
  3. Abe, N., Wu, Z. & Yoshikawa, H. ( 2003b; ). Molecular characterization of Blastocystis isolates from primates. Vet Parasitol 113, 321–325.[CrossRef]
    [Google Scholar]
  4. Abe, N., Wu, Z. & Yoshikawa, H. ( 2003c; ). Zoonotic genotypes of Blastocystis hominis detected in cattle and pigs by PCR with diagnostic primers and restriction fragment length polymorphism analysis of the small subunit ribosomal RNA gene. Parasitol Res 90, 124–128.
    [Google Scholar]
  5. Arisue, N., Hashimoto, T. & Yoshikawa, H. ( 2003; ). Sequence heterogeneity of the small subunit ribosomal RNA genes among Blastocystis isolates. Parasitology 126, 1–9.[CrossRef]
    [Google Scholar]
  6. Belova, L. M. ( 1991; ). Blastocystis anatis sp. nov. (Rhizopoda, Lobosea) from Anas platyrhynchos. Zool Zh 70, 5–10 (in Russian).
    [Google Scholar]
  7. Belova, L. M. ( 1995; ). Blastocystis fauna. Parazitologiia 29, 208–213 (in Russian).
    [Google Scholar]
  8. Belova, L. M. & Kostenko, L. A. ( 1990; ). Blastocystis galli sp. n. (Protista: Rhizopoda) from the intestine of domestic hens. Parazitologiia 24, 164–168 (in Russian).
    [Google Scholar]
  9. Brumpt, E. ( 1912; ). Blastocystis hominis n. sp. et formes voisines. Bull Soc Pathol Exot 5, 725–730.
    [Google Scholar]
  10. Burden, D. J., Anger, H. S. & Hammet, N. C. ( 1978/1979; ). Blastocystis sp. infections in pigs. Vet Microbiol 3, 227–234.
    [Google Scholar]
  11. Carbajal, J. A., Del Castillo, L., Lanuza, M. D., Villar, J. & Borras, R. ( 1997; ). Karyotypic diversity among Blastocystis hominis isolates. Int J Parasitol 27, 941–945.[CrossRef]
    [Google Scholar]
  12. Chen, X. Q., Singh, M., Ho, L. C., Tan, S. W., Ng, G. C., Moe, K. T. & Yap, E. H. ( 1997; ). Description of a Blastocystis species from Rattus norvegicus. Parasitol Res 83, 313–318.[CrossRef]
    [Google Scholar]
  13. Clark, C. G. ( 1997; ). Extensive genetic diversity in Blastocystis hominis. Mol Biochem Parasitol 87, 79–83.[CrossRef]
    [Google Scholar]
  14. Krylov, M. V. & Belova, L. M. ( 1997; ). Blastocystis from primates. Parazitologiia 31, 341–345 (in Russian).
    [Google Scholar]
  15. Noël, C., Peyronnet, C., Gerbod, D., Edgcomb, V. P., Delgado-Viscogliosi, P., Sogin, M. L., Capron, M., Viscogliosi, E. & Zenner, L. ( 2003; ). Phylogenetic analysis of Blastocystis isolates from different hosts based on the comparison of small-subunit rRNA gene sequences. Mol Biochem Parasitol 126, 119–123.[CrossRef]
    [Google Scholar]
  16. Pakandl, M. ( 1991; ). Occurrence of Blastocystis sp. in pigs. Folia Parasitol 38, 297–301.
    [Google Scholar]
  17. Quílez, J., Sánchez-Acedo, C., Clavel, A. & Causapé, A. C. ( 1995; ). Occurrence of Blastocystis sp. in cattle in Aragón, northeastern Spain. Parasitol Res 81, 703–705.[CrossRef]
    [Google Scholar]
  18. Thathaisong, U., Worapong, J., Mungthin, M., Tan-Ariya, P., Viputtigul, K., Sudatis, A., Noonai, A. & Leelayoova, S. ( 2003; ). Blastocystis isolates from a pig and a horse are closely related to Blastocystis hominis. J Clin Microbiol 41, 967–975.[CrossRef]
    [Google Scholar]
  19. Upcroft, J. A., Dunn, L. A., Dommett, L. S., Healey, A., Upcroft, P. & Boreham, P. F. L. ( 1989; ). Chromosomes of Blastocystis hominis. Int J Parasitol 19, 879–883.[CrossRef]
    [Google Scholar]
  20. Windsor, J. J., Macfarlane, L., Hughes-Thapa, G., Jones, S. K. A. & Whiteside, T. M. ( 2002; ). Incidence of Blastocystis hominis in faecal samples submitted for routine microbiological analysis. Br J Biomed Sci 59, 154–157.
    [Google Scholar]
  21. Yoshikawa, H., Nagano, I., Yap, E. H., Singh, M. & Takahashi, Y. ( 1996; ). DNA polymorphism revealed by arbitrary primers polymerase chain reaction among Blastocystis strains isolated from humans, a chicken, and a reptile. J Eukaryot Microbiol 43, 127–130.[CrossRef]
    [Google Scholar]
  22. Yoshikawa, H., Nagano, I., Wu, Z., Yap, E. H., Singh, M. & Takahashi, Y. ( 1998; ). Genomic polymorphism among Blastocystis strains and development of subtype-specific diagnostic primers. Mol Cell Probes 12, 153–159.[CrossRef]
    [Google Scholar]
  23. Yoshikawa, H., Abe, N., Iwasawa, M., Kitano, S., Nagano, I., Wu, Z. & Takahashi, Y. ( 2000; ). Genomic analysis of Blastocystis hominis strains isolated from two long-term health care facilities. J Clin Microbiol 38, 1324–1330.
    [Google Scholar]
  24. Yoshikawa, H., Wu, Z., Nagano, I. & Takahashi, Y. ( 2003; ). Molecular comparative studies among Blastocystis isolates obtained from humans and animals. J Parasitol 89, 585–594.[CrossRef]
    [Google Scholar]
  25. Yoshikawa, H., Wu, Z., Kimata, I., Iseki, M., Karim, M. D. A. I., Hossain, M. B., Zaman, V., Haque, R. & Takahashi, Y. ( 2004; ). Polymerase chain reaction-based genotype classification among human Blastocystis hominis populations isolated from different countries. Parasitol Res 92, 22–29.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.26899-0
Loading
/content/journal/micro/10.1099/mic.0.26899-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error