1887

Abstract

In this study, the identification and characterization of the homologues of -1,6-mannosyltransferases Anp1p and Och1p, designated YlAnl1p and YlOch1p, are described. In order to confirm the function of the proteins, including the previously isolated YlMnn9p, in the -glycosylation pathway, a phenotypic analysis of the disrupted strains Δ, Δ, Δ, ΔΔ and ΔΔ was performed. Disruption of the , and genes caused an increased sensitivity to SDS, compatible with a glycosylation defect, and to Calcofluor White, characteristic of cell-wall defects. Moreover, Western-blot analysis of a heterologous glycosylated protein confirmed a direct role of YlMnn9p and YlAnl1p in the -glycosylation process. These mutant strains, Δ, Δ, Δ, ΔΔ and ΔΔ may thus be used to establish a model for the -linked glycosylation pathway.

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2004-07-01
2019-11-21
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