1887

Abstract

The gene encodes NADH dehydrogenase II, a primary dehydrogenase used during aerobic and nitrate respiration. The anaerobic transcription factor FNR represses expression by binding at two sites centred at −94·5 and −50·5. transcription studies using promoter fusions with 5′ deletions confirmed that both FNR sites are required for maximum repression under anaerobic conditions. The histone-like protein Fis binds to three sites [centred at −123 (Fis I), −72, (Fis II) and +51 (Fis III)] in the promoter. Using  : :  promoter fusions carrying 5′ deletions, or replacement mutations it is shown that Fis III is a repressing site and that Fis I and II are activating sites, with the greatest contribution from Fis II. Deletion of the C-terminal domain of the RNA polymerase -subunit abolished Fis-mediated activation of expression, suggesting that has a Class I Fis-activated promoter. In accordance with the established pattern of Fis synthesis, transcription was greatest during exponential growth. Thus, it is suggested that Fis enhances expression during periods of rapid growth, by acting as a Class I activator, and that the binding of tandem FNR dimers represses expression by preventing interaction of the RNA polymerase -subunit with DNA and Fis.

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2004-02-01
2020-01-20
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