1887

Abstract

is an anoxygenic photosynthetic bacterium that is capable of differentiating into several cell types. When grown phototrophically in liquid, cells exhibit a vibrioid shape and have a single polar flagellum. When grown on a solid surface, will differentiate into rod-shaped swarm cells that display numerous lateral flagella. Upon starvation for nutrients, also forms desiccation-resistant cysts. In this study, it was determined that has heat- and desiccation-resistance properties similar to other cyst-forming species. In addition, microscopic analyses of the morphological changes that occur during cyst cell development were performed. It was observed that typically forms multi-celled clusters of cysts that contain from four to more than 10 cells per cluster. It was also determined that cell density has a minor effect on the percentage of cyst cells formed, with cell densities of 10–10 cells per 5 μl spot yielding the highest percentage of cyst cells. The striking similarities between the life cycle of and the life cycle exhibited by spp. are discussed.

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2004-02-01
2020-08-12
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References

  1. Bisset K. A. 1973; The zonation phenomenon and structure of the swarm colony in Proteus mirabilis . J Med Microbiol6:429–433[CrossRef]
    [Google Scholar]
  2. Branda S. S., Gonzalez-Pastor J. E., Ben-Yehuda S., Losick R., Kolter R. 2001; Fruiting body formation by Bacillus subtilis . Proc Natl Acad Sci U S A98:11621–11626[CrossRef]
    [Google Scholar]
  3. Cagle G. D., Vela G. R. 1972; Cysts of Azotobacter vinelandii with double coats. J Bacteriol112:615–617
    [Google Scholar]
  4. Dworkin M. 2000; Introduction to the Myxobacteria . In Prokaryotic Development pp221–242 Edited by Brun Y., Shimkets L.. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  5. Favinger J., Stadtwald R., Gest H. 1989; Rhodospirillum centenum , sp. nov., a thermotolerant cyst-forming anoxygenic photosynthetic bacterium. Antonie Van Leeuwenhoek55:291–296[CrossRef]
    [Google Scholar]
  6. Grossman A. D., Losick R. 1988; Extracellular control of spore formation in Bacillus subtilis . Proc Natl Acad Sci U S A85:4369–4373[CrossRef]
    [Google Scholar]
  7. Jiang Z. Y., Rushing B. G., Bai Y., Gest H., Bauer C. E. 1998; Isolation of Rhodospirillum centenum mutants defective in phototactic colony motility by transposon mutagenesis. J Bacteriol180:1248–1255
    [Google Scholar]
  8. Kaplan H. B., Plamann L. 1996; A Myxococcus xanthus cell density-sensing system required for multicellular development. FEMS Microbiol Lett139:89–95[CrossRef]
    [Google Scholar]
  9. Katupitiya S., Millet J., Vesk M., Viccars L., Zeman A., Lidong Z., Elmerich C., Kennedy I. R. 1995; A mutant of Azospirillum brasilense Sp7 impaired in flocculation with a modified colonization pattern and superior nitrogen fixation in association with wheat. Appl Environ Microbiol61:1987–1995
    [Google Scholar]
  10. McClain J., Rollo D. R., Rushing B. G., Bauer C. E. 2002; Rhodospirillum centenum utilizes separate motor and switch components to control lateral and polar flagellum rotation. J Bacteriol184:2429–2438[CrossRef]
    [Google Scholar]
  11. Moens S., Schloter M., Vanderleyden J. 1996; Expression of the structural gene, laf1 , encoding the flagellin of the lateral flagella in Azospirillum brasilense Sp7. J Bacteriol178:5017–5019
    [Google Scholar]
  12. Nicholson W. L., Fajardo-Cavazos P., Rebeil R., Slieman T. A., Riesenman P. J., Law J. F., Xue Y. 2002; Bacterial endospores and their significance in stress resistance. Antonie Van Leeuwenhoek81:27–32[CrossRef]
    [Google Scholar]
  13. Nickens D., Fry C., Ragatz L., Bauer C. E., Gest H. 1996; Biotype of the purple nonsulfur photosynthetic bacterium, Rhodospirillum centenum . Arch Microbiol165:91–96[CrossRef]
    [Google Scholar]
  14. Okon Y., Itzigsohn R. 1995; The development of Azospirillum as a commercial inoculant for improving crop yields. Biotechnol Adv13:415–424[CrossRef]
    [Google Scholar]
  15. Pereg Gerk L., Gilchrist K., Kennedy I. R. 2000; Mutants with enhanced nitrogenase activity in hydroponic Azospirillum brasilense -wheat associations. Appl Environ Microbiol66:2175–2184[CrossRef]
    [Google Scholar]
  16. Pope L. M., Wyss O. 1970; Outer layers of the Azotobacter vinelandii cyst. J Bacteriol102:234–239
    [Google Scholar]
  17. Ragatz L., Jiang Z. Y., Bauer C. E., Gest H. 1995; Macroscopic phototactic behavior of the purple photosynthetic bacterium Rhodospirillum centenum . Arch Microbiol163:1–6[CrossRef]
    [Google Scholar]
  18. Sadasivan L., Neyra C. A. 1985; Flocculation in Azospirillum brasilense and Azospirillum lipoferum : exopolysaccharides and cyst formation. J Bacteriol163:716–723
    [Google Scholar]
  19. Sadasivan L., Neyra C. A. 1987; Cyst production and brown pigment formation in aging cultures of Azospirillum brasilense ATCC 29145. J Bacteriol169:1670–1677
    [Google Scholar]
  20. Sadoff H. L. 1973; Comparative aspects of morphogenesis in three prokaryotic genera. Annu Rev Microbiol27:133–153[CrossRef]
    [Google Scholar]
  21. Sadoff H. L. 1975; Encystment and germination in Azotobacter vinelandii . Bacteriol Rev39:516–539
    [Google Scholar]
  22. Scheludko A. V., Katsy E. I., Ostudin N. A., Gringauz O. K., Panasenko V. I. 1998; Novel classes of Azospirillum brasilense mutants with defects in the assembly and functioning of polar and lateral flagella. Mol Gen Mikrobiol Virusol(4:33–37
    [Google Scholar]
  23. Socolofsky M. D., Wyss O. 1962; Resistance of the Azotobacter cyst. J Bacteriol84:119–124
    [Google Scholar]
  24. Sonenshein A. 2000; Endospore-forming bacteria: an overview. In Prokaryotic Development pp133–150 Edited by Brun Y., Shimkets L.. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  25. Stadtwald-Demchick R., Turner F. R., Gest H. 1990; Physiological properties of the thermotolerant photosynthetic bacterium, Rhodospirillum centenum . FEMS Microbiol Lett67:139–144[CrossRef]
    [Google Scholar]
  26. Steenhoudt O., Vanderleyden J. 2000; Azospirillum , a free-living nitrogen-fixing bacterium closely associated with grasses: genetic, biochemical and ecological aspects. FEMS Microbiol Rev24:487–506[CrossRef]
    [Google Scholar]
  27. Stephenson K., Hoch J. A. 2002; Evolution of signalling in the sporulation phosphorelay. Mol Microbiol46:297–304[CrossRef]
    [Google Scholar]
  28. Stevenson L. H., Socolofsky M. D. 1966; Cyst formation and poly-beta-hydroxybutyric acid accumulation in Azotobacter . J Bacteriol91:304–310
    [Google Scholar]
  29. Stevenson L. H., Socolofsky M. D. 1973; Role of poly-beta-hydroxybutyric acid in cyst formation by Azotobacter . Antonie Van Leeuwenhoek39:341–350[CrossRef]
    [Google Scholar]
  30. Stoffels M., Castellanos T., Hartmann A. 2001; Design and application of new 16S rRNA-targeted oligonucleotide probes for the Azospirillum - Skermanella - Rhodocista -cluster. Syst Appl Microbiol24:83–97[CrossRef]
    [Google Scholar]
  31. Takamatsu H., Watabe K. 2002; Assembly and genetics of spore protective structures. Cell Mol Life Sci59:434–444[CrossRef]
    [Google Scholar]
  32. Tarrand J. J., Krieg N. R., Dobereiner J. 1978; A taxonomic study of the Spirillum lipoferum group, with descriptions of a new genus, Azospirillum gen. nov. and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov. Can J Microbiol24:967–980[CrossRef]
    [Google Scholar]
  33. Tudor J. J., Conti S. F. 1977; Characterization of bdellocysts of Bdellovibrio sp. J Bacteriol131:314–322
    [Google Scholar]
  34. Whittenbury R., Davies S. L., Davey J. F. 1970; Exospores and cysts formed by methane-utilizing bacteria. J Gen Microbiol61:219–226[CrossRef]
    [Google Scholar]
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