1887

Abstract

Previously, the authors have shown that inactivation of , a gene of unknown function upstream of , which encodes a periplasmic disulphide catalyst, results in a global change of gene expression. Among the severely down-regulated genes are , suggesting that the mutant, Sh54, may inefficiently produce the UDP-glucose and UDP-galactose required for LPS synthesis. This paper demonstrates that LPS synthesis in Sh54 is impaired. As a result, Sh54 is unable to polymerize host cell actin, due to aberrant localization of IcsA, or to cause keratoconjunctivitis in guinea pigs. Furthermore, Sh54 is more sensitive to some antimicrobial agents, and exhibits epithelial cytotoxicity characteristic of neither wild-type nor mutants. Supplying restores LPS synthesis and corrects all the defects. Hence, it is clear that the gene is important not only in regulating global gene expression, as shown previously, but also in virulence through LPS synthesis via regulating the expression of the operon.

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2004-04-01
2024-03-29
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