1887

Abstract

The plasminogen activator streptokinase has been proposed to be a key component of a complex mechanism that promotes skin invasion by . This study was designed to compare gene message and protein levels in wild-type M1 serotype isolate 1881 and a more invasive variant recovered from the spleen of a lethally infected mouse. M1 isolates selected for invasiveness demonstrated enhanced levels of active plasminogen activator activity in culture. This effect was due to a combination of increased expression of the gene and decreased expression of the gene. The gene product, SpeB, was found to efficiently degrade streptokinase .

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2004-02-01
2024-04-23
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References

  1. Berge A., Kihlberg B. M., Sjoholm A. G., Bjorck L. 1997; Streptococcal protein H forms soluble complement-activating complexes with IgG, but inhibits complement activation by IgG-coated targets. J Biol Chem 272:20774–20781 [CrossRef]
    [Google Scholar]
  2. Boyle M. D., Lottenberg R. 1997; Plasminogen activation by invasive human pathogens. Thromb Haemost 77:1–10
    [Google Scholar]
  3. Boyle M. D., Raeder R., Flosdorff A., Podbielski A. 1998; Role of emm and mrp genes in the virulence of group A streptococcal isolate 64/14 in a mouse model of skin infection. J Infect Dis 177:991–997 [CrossRef]
    [Google Scholar]
  4. Boyle M. D., Romer T. G., Meeker A. K., Sledjeski D. D. 2001; Use of surface-enhanced laser desorption ionization protein chip system to analyze streptococcal exotoxin B activity secreted by Streptococcus pyogenes . J Microbiol Methods 46:87–97 [CrossRef]
    [Google Scholar]
  5. Caparon M. G., Scott J. R. 1987; Identification of a gene that regulates expression of M protein, the major virulence determinant of group A streptococci. Proc Natl Acad Sci U S A 84:8677–8681 [CrossRef]
    [Google Scholar]
  6. Chaussee M. S., Ajdic D., Ferretti J. J. 1999; The rgg gene of Streptococcus pyogenes NZ131 positively influences extracellular SPE B production. Infect Immun 67:1715–1722
    [Google Scholar]
  7. Chaussee M. S., Watson R. O., Smoot J. C., Musser J. M. 2001; Identification of Rgg-regulated exoproteins of Streptococcus pyogenes . Infect Immun 69:822–831 [CrossRef]
    [Google Scholar]
  8. Christner R., Li Z., Raeder R., Podbielski A., Boyle M. D. 1997; Identification of key gene products required for acquisition of plasmin-like enzymatic activity by group A streptococci. J Infect Dis 175:1115–1120 [CrossRef]
    [Google Scholar]
  9. Coleman J. L., Benach J. L. 1999; Use of the plasminogen activation system by microorganisms. J Lab Clin Med 134:567–576 [CrossRef]
    [Google Scholar]
  10. D’Costa S. S., Boyle M. D. 1998; Interaction of a group A streptococcus within human plasma results in assembly of a surface plasminogen activator that contributes to occupancy of surface plasmin-binding structures. Microb Pathog 24:341–349 [CrossRef]
    [Google Scholar]
  11. D’Costa S. S., Boyle M. D. 2000; Interaction of group A streptococci with human plasmin(ogen) under physiological conditions. Methods 21:165–177 [CrossRef]
    [Google Scholar]
  12. D’Costa S. S., Romer T. G., Boyle M. D. 2000; Analysis of expression of a cytosolic enzyme on the surface of Streptococcus pyogenes . Biochem Biophys Res Commun 278:826–832 [CrossRef]
    [Google Scholar]
  13. DesJardin L. E., Boyle M. D., Lottenberg R. 1989; Group A streptococci bind human plasmin but not other structurally related proteins. Thromb Res 55:187–193 [CrossRef]
    [Google Scholar]
  14. Federle M. J., McIver K. S., Scott J. R. 1999; A response regulator that represses transcription of several virulence operons in the group A streptococcus. J Bacteriol 181:3649–3657
    [Google Scholar]
  15. Ferretti J. J., McShan W. M., Ajdic D. 20 other authors 2001; Complete genome sequence of an M1 strain of Streptococcus pyogenes . Proc Natl Acad Sci U S A 98:4658–4663 [CrossRef]
    [Google Scholar]
  16. Heath A., DiRita V. J., Barg N. L., Engleberg N. C. 1999; A two-component regulatory system, CsrR-CsrS, represses expression of three Streptococcus pyogenes virulence factors, hyaluronic acid capsule, streptolysin S, and pyrogenic exotoxin B. Infect Immun 67:5298–5305
    [Google Scholar]
  17. Johnston K. H., Zabriskie J. B. 1986; Purification and partial characterization of the nephritis strain-associated protein from Streptococcus pyogenes , group A. J Exp Med 163:697–712 [CrossRef]
    [Google Scholar]
  18. Kreikemeyer B., Boyle M. D., Buttaro B. A., Heinemann M., Podbielski A. 2001; Group A streptococcal growth phase-associated virulence factor regulation by a novel operon (Fas) with homologies to two-component-type regulators requires a small RNA molecule. Mol Microbiol 39:392–406 [CrossRef]
    [Google Scholar]
  19. Lahteenmaki K., Kuusela P., Korhonen T. K. 2001; Bacterial plasminogen activators and receptors. FEMS Microbiol Rev 25:531–552 [CrossRef]
    [Google Scholar]
  20. Lei B., Mackie S., Lukomski S., Musser J. M. 2000; Identification and immunogenicity of group A Streptococcus culture supernatant proteins. Infect Immun 68:6807–6818 [CrossRef]
    [Google Scholar]
  21. Li Z., Ploplis V. A., French E. L., Boyle M. D. 1999a; Interaction between group A streptococci and the plasmin(ogen) system promotes virulence in a mouse skin infection model. J Infect Dis 179:907–914 [CrossRef]
    [Google Scholar]
  22. Li Z., Sledjeski D. D., Kreikemeyer B., Podbielski A., Boyle M. D. 1999b; Identification of pel , a Streptococcus pyogenes locus that affects both surface and secreted proteins. J Bacteriol 181:6019–6027
    [Google Scholar]
  23. Lottenberg R., Broder C. C., Boyle M. D. 1987; Identification of a specific receptor for plasmin on a group A streptococcus. Infect Immun 55:1914–1918
    [Google Scholar]
  24. Lottenberg R., Minning-Wenz D., Boyle M. D. 1994; Capturing host plasmin(ogen): a common mechanism for invasive pathogens?. Trends Microbiol 2:20–24 [CrossRef]
    [Google Scholar]
  25. Lyon W. R., Gibson C. M., Caparon M. G. 1998; A role for trigger factor and an rgg -like regulator in the transcription, secretion and processing of the cysteine proteinase of Streptococcus pyogenes . EMBO J 17:6263–6275 [CrossRef]
    [Google Scholar]
  26. Lyon W. R., Madden J. C., Levin J. C., Stein J. L., Caparon M. G. 2001; Mutation of luxS affects growth and virulence factor expression in Streptococcus pyogenes . Mol Microbiol 42:145–157
    [Google Scholar]
  27. Marcum J. A., Kline D. L. 1983; Species specificity of streptokinase. Comp Biochem Physiol B 75:389–394
    [Google Scholar]
  28. McCoy H. E., Broder C. C., Lottenberg R. 1991; Streptokinases produced by pathogenic group C streptococci demonstrate species-specific plasminogen activation. J Infect Dis 164:515–521 [CrossRef]
    [Google Scholar]
  29. McIver K. S., Scott J. R. 1997; Role of mga in growth phase regulation of virulence genes of the group A streptococcus. J Bacteriol 179:5178–5187
    [Google Scholar]
  30. Nowicki S. T., Minning-Wenz D., Johnston K. H., Lottenberg R. 1994; Characterization of a novel streptokinase produced by Streptococcus equisimilis of non-human origin. Thromb Haemost 72:595–603
    [Google Scholar]
  31. Pinkney M., Kapur V., Smith J. 7 other authors 1995; Different forms of streptolysin O produced by Streptococcus pyogenes and by Escherichia coli expressing recombinant toxin: cleavage by streptococcal cysteine protease. Infect Immun 63:2776–2779
    [Google Scholar]
  32. Radek J. T., Castellino F. J. 1989; Conformational properties of streptokinase. J Biol Chem 264:9915–9922
    [Google Scholar]
  33. Raeder R., Boyle M. D. 1993; Association between expression of immunoglobulin G-binding proteins by group A streptococci and virulence in a mouse skin infection model. Infect Immun 61:1378–1384
    [Google Scholar]
  34. Raeder R., Boyle M. D. 1995; Distinct profiles of immunoglobulin G-binding-protein expression by invasive serotype M1 isolates of Streptococcus pyogenes . Clin Diagn Lab Immunol 2:478–483
    [Google Scholar]
  35. Raeder R., Boyle M. D. 1996; Properties of IgG-binding proteins expressed by Streptococcus pyogenes isolates are predictive of invasive potential. J Infect Dis 173:888–895 [CrossRef]
    [Google Scholar]
  36. Raeder R., Woischnik M., Podbielski A., Boyle M. D. 1998; A secreted streptococcal cysteine protease can cleave a surface-expressed M1 protein and alter the immunoglobulin binding properties. Res Microbiol 149:539–548 [CrossRef]
    [Google Scholar]
  37. Raeder R., Harokopakis E., Hollingshead S., Boyle M. D. 2000; Absence of SpeB production in virulent large capsular forms of group A streptococcal strain 64. Infect Immun 68:744–751 [CrossRef]
    [Google Scholar]
  38. Robbins K. C., Markus G. 1976; The interaction of human plasminogen with streptokinase. In Fibrinolysis pp 61–75 Edited by Gaffney P. J., Balkuv-Ulutin S. New York: Academic Press;
    [Google Scholar]
  39. Saouda M., Wu W., Conran P., Boyle M. D. 2001; Streptococcal pyrogenic exotoxin B enhances tissue damage initiated by other Streptococcus pyogenes products. J Infect Dis 184:723–731 [CrossRef]
    [Google Scholar]
  40. Schroeder B., Boyle M. D., Sheerin B. R., Asbury A. C., Lottenberg R. 1999; Species specificity of plasminogen activation and acquisition of surface-associated proteolytic activity by group C streptococci grown in plasma. Infect Immun 67:6487–6495
    [Google Scholar]
  41. Sledjeski D. D., Coco C., Corriher C., Boyle M. D. 2001; Isolation of human plasma-inducible, growth phase- and temperature-regulated gene fusions in Streptococcus pyogenes using a Tn 917 - lacZ transposon. J Microbiol Methods 46:107–117 [CrossRef]
    [Google Scholar]
  42. Smith T. C., Sledjeski D. D., Boyle M. D. 2003a; Regulation of protein H expression in M1 serotype isolates of Streptococcus pyogenes . FEMS Microbiol Lett 219:9–15 [CrossRef]
    [Google Scholar]
  43. Smith T. C., Sledjeski D. D., Boyle M. D. 2003b; Streptococcus pyogenes infection in mouse skin leads to a time-dependent up-regulation of protein H expression. Infect Immun 71:6079–6082 [CrossRef]
    [Google Scholar]
  44. Svensson M. D., Sjobring U., Luo F., Bessen D. E. 2002; Roles of the plasminogen activator streptokinase and the plasminogen-associated M protein in an experimental model for streptococcal impetigo. Microbiology 148:3933–3945
    [Google Scholar]
  45. Talkington D. F., Schwartz B., Black C. M., Todd J. K., Elliott J., Breiman R. F., Facklam R. R. 1993; Association of phenotypic and genotypic characteristics of invasive Streptococcus pyogenes isolates with clinical components of streptococcal toxic shock syndrome. Infect Immun 61:3369–3374
    [Google Scholar]
  46. Wang H., Lottenberg R., Boyle M. D. 1994; Analysis of plasmin(ogen) acquisition by clinical isolates of group A streptococci incubated in human plasma. J Infect Dis 169:143–149 [CrossRef]
    [Google Scholar]
  47. Wang H., Lottenberg R., Boyle M. D. 1995a; Analysis of the interaction of group A streptococci with fibrinogen, streptokinase and plasminogen. Microb Pathog 18:153–166 [CrossRef]
    [Google Scholar]
  48. Wang H., Lottenberg R., Boyle M. D. 1995b; A role for fibrinogen in the streptokinase-dependent acquisition of plasmin(ogen) by group A streptococci. J Infect Dis 171:85–92 [CrossRef]
    [Google Scholar]
  49. Wohl R. C., Sinio L., Summaria L., Robbins K. C. 1983; Comparative activation kinetics of mammalian plasminogens. Biochim Biophys Acta 745:20–31 [CrossRef]
    [Google Scholar]
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