1887

Abstract

is a major aetiological organism implicated in periodontal disease. The interaction of with other oral bacteria, in particular , in biofilm formation is thought to be an important step in the onset of periodontal disease. The interaction between and has been examined using a panel of mutants and their effects on mixed biofilm formation tested in a static biofilm model. ATCC 35405 did not form detectable biofilms on various inert surfaces. However, the spirochaete was demonstrated to form a biofilm with preattached 381. , which lacks the cytoplasmic filament, was unable to produce a mixed biofilm with . A mutant which lacks the flagella hook protein and is therefore non-motile displayed a reduced, but readily detectable, ability to form a mixed biofilm as did the mutant which does not possess the major outer sheath protein (Msp). The mutant was only moderately defective in forming mixed biofilms with . However, the methyl-accepting chemotaxis protein (DmcA) did not appear to play a major role in mixed biofilm formation. In contrast, lacking the PrtP protein for prolyl-phenylalanine-specific protease, showed an increased ability to form mixed biofilms and a prolonged viability in the biofilm.

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2004-07-01
2024-11-14
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References

  1. Arakawa S., Kuramitsu H. K. 1994; Cloning and sequence analysis of a chymotrypsin like protease from Treponema denticola. Infect Immun 62:3424–3433
    [Google Scholar]
  2. Asai Y., Jinno T., Igarashi H., Ohyama Y., Ogawa T. 2002; Detection and quantification of oral treponemes in subgingival plaque by real time PCR. J Clin Microbiol 40:3334–3340 [CrossRef]
    [Google Scholar]
  3. Dawson J. R., Ellen R. P. 1994; Clustering of fibronectin adhesins towards Treponema denticola tips upon contact with immobilized fibronectin. Infect Immun 62:2214–2221
    [Google Scholar]
  4. Fenno J. C., Muller K. H., McBride B. C. 1996; Sequence analysis, expression, and binding activity of recombinant major outer sheath protein (Msp) of Treponema denticola. J Bacteriol 178:2489–2497
    [Google Scholar]
  5. Fenno J. C., Wong G. L., Hannam P. M., McBride B. C. 1998; Mutagenesis of outer membrane virulence determinants of the oral spirochete Treponema denticola. FEMS Microbiol Lett 163:209–215 [CrossRef]
    [Google Scholar]
  6. Grenier D. 1992; Nutritional interactions between two suspected periodontopathogens, Treponema denticola and Porphyromonas gingivalis. Infect Immun 60:5298–5301
    [Google Scholar]
  7. Haffajee A. D., Socransky S. S. 1994; Microbial etiological agents of destructive periodontal diseases. Periodontol 2000; 5:78–111 [CrossRef]
    [Google Scholar]
  8. Ishihara K., Miura T., Kuramitsu H. K., Okuda K. 1996; Characterization of the Treponema denticola prtP gene encoding a prolyl-phenyalanine-specific protease (dentilisin. Infect Immun 64:5178–5186
    [Google Scholar]
  9. Ishihara K., Kuramitsu H. K., Miura T., Okuda K. 1998; Dentilisin activity affects the organization of the outer sheath of Treponema denticola. J Bacteriol 180:3837–3844
    [Google Scholar]
  10. Izard J., Samsonoff W. A., Limberger R. J. 2001; Cytoplasmic filament-deficient mutant of Treponema denticola has pleiotropic defects. J Bacteriol 183:1078–1084 [CrossRef]
    [Google Scholar]
  11. Kataoka M., Li H., Arakawa S., Kuramitsu H. 1997; Characterization of a methyl-accepting chemotaxis protein gene, dmcA, from the oral spirochete Treponema denticola. Infect Immun 65:4011–4016
    [Google Scholar]
  12. Kigure T., Saito A., Seida K., Yamada S., Ishihara K., Okuda K. 1995; Distribution of Porphyromonas gingivalis and Treponema denticola in human subgingival plaque at different periodontal pocket depths examined by immunochemical methods. J Periodontol Res 30:332–341 [CrossRef]
    [Google Scholar]
  13. Kimsey R. B., Spielman A. 1990; Motility of Lyme disease spirochetes in fluids as viscous as the extracellular matrix. J Infect Dis 162:1205–1208 [CrossRef]
    [Google Scholar]
  14. Klitorinos A., Noble P., Siboo R., Chan E. C. 1993; Viscosity-dependent locomotion of oral spirochetes. Oral Microbiol Immunol 8:242–244 [CrossRef]
    [Google Scholar]
  15. Kolenbrander P. E. 1998; Intergeneric coaggregation among human oral bacteria and ecology of dental plaque. Annu Rev Microbiol 42:627–656
    [Google Scholar]
  16. Kolenbrander P. E., Roxannan A., Blehert D. S., Egland P. G., Foster J. S., Palmer R. J. Jr 2002; Communication among oral bacteria. Microbiol Mol Biol Rev 66:486–505 [CrossRef]
    [Google Scholar]
  17. Leung W. K., Haapasalo M., Uitto V.-J., Hannam P. M., McBride B. C. 1996; The surface proteinase of Treponema denticola may mediate attachment of the bacteria to epithelial cells. Anaerobe 2:39–46 [CrossRef]
    [Google Scholar]
  18. Li H., Ruby J., Charon N., Kuramitsu H. 1996; Gene inactivation in the oral spirochete Treponema denticola: construction of an flgE mutant. J Bacteriol 178:3664–3667
    [Google Scholar]
  19. Limberger R. J., Slivienski L. L., Izard J., Samsonoff W. A. 1999; Insertional inactivation of Treponema denticola tap1 results in a nonmotile mutant with elongated flagellar hooks. J Bacteriol 181:3743–3750
    [Google Scholar]
  20. Loesche W. J. 1988 The role of spirochetes in periodontal disease Adv Dent Res;275–283
    [Google Scholar]
  21. Loesche W. J. 1993; Bacterial mediators in periodontal disease. Clin Infect Dis 16 (suppl. 44:S203–S210
    [Google Scholar]
  22. O'Toole G. A., Kaplan H. B., Kolter R. 2000; Biofilm formation as microbial development. Annu Rev Microbiol 54:49–79 [CrossRef]
    [Google Scholar]
  23. Pratt L. A., Kolter P. 1998; Genetic analysis of Escherichia coli biofilm formation: roles of flagella, motility, chemotaxis and type I pili. Mol Microbiol 30:285–293 [CrossRef]
    [Google Scholar]
  24. Saglie R., Newman M. G., Carranza F. A., Pattison G. L. 1982; Bacterial invasion of gingiva in advanced periodontitis in humans. J Periodontol 53:217–222 [CrossRef]
    [Google Scholar]
  25. Simonson L. G., Goodman C. H., Bial J. J., Morton H. E. 1988; Quantitative relationship of Treponema denticola to severity of periodontal disease. Infect Immun 56:726–728
    [Google Scholar]
  26. Slots J., Genco R. J. 1984; Black-pigmented Bacteroides species, Capnocytophaga species, and Actinobacillus actinomycetemcomitans in human periodontal disease: virulence factors in colonization, survival, and tissue destruction. J Dent Res 63:412–421 [CrossRef]
    [Google Scholar]
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