1887

Abstract

is a major aetiological organism implicated in periodontal disease. The interaction of with other oral bacteria, in particular , in biofilm formation is thought to be an important step in the onset of periodontal disease. The interaction between and has been examined using a panel of mutants and their effects on mixed biofilm formation tested in a static biofilm model. ATCC 35405 did not form detectable biofilms on various inert surfaces. However, the spirochaete was demonstrated to form a biofilm with preattached 381. , which lacks the cytoplasmic filament, was unable to produce a mixed biofilm with . A mutant which lacks the flagella hook protein and is therefore non-motile displayed a reduced, but readily detectable, ability to form a mixed biofilm as did the mutant which does not possess the major outer sheath protein (Msp). The mutant was only moderately defective in forming mixed biofilms with . However, the methyl-accepting chemotaxis protein (DmcA) did not appear to play a major role in mixed biofilm formation. In contrast, lacking the PrtP protein for prolyl-phenylalanine-specific protease, showed an increased ability to form mixed biofilms and a prolonged viability in the biofilm.

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2004-07-01
2019-10-23
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References

  1. Arakawa, S. & Kuramitsu, H. K. ( 1994; ). Cloning and sequence analysis of a chymotrypsin like protease from Treponema denticola. Infect Immun 62, 3424–3433.
    [Google Scholar]
  2. Asai, Y., Jinno, T., Igarashi, H., Ohyama, Y. & Ogawa, T. ( 2002; ). Detection and quantification of oral treponemes in subgingival plaque by real time PCR. J Clin Microbiol 40, 3334–3340.[CrossRef]
    [Google Scholar]
  3. Dawson, J. R. & Ellen, R. P. ( 1994; ). Clustering of fibronectin adhesins towards Treponema denticola tips upon contact with immobilized fibronectin. Infect Immun 62, 2214–2221.
    [Google Scholar]
  4. Fenno, J. C., Muller, K. H. & McBride, B. C. ( 1996; ). Sequence analysis, expression, and binding activity of recombinant major outer sheath protein (Msp) of Treponema denticola. J Bacteriol 178, 2489–2497.
    [Google Scholar]
  5. Fenno, J. C., Wong, G. L., Hannam, P. M. & McBride, B. C. ( 1998; ). Mutagenesis of outer membrane virulence determinants of the oral spirochete Treponema denticola. FEMS Microbiol Lett 163, 209–215.[CrossRef]
    [Google Scholar]
  6. Grenier, D. ( 1992; ). Nutritional interactions between two suspected periodontopathogens, Treponema denticola and Porphyromonas gingivalis. Infect Immun 60, 5298–5301.
    [Google Scholar]
  7. Haffajee, A. D. & Socransky, S. S. ( 1994; ). Microbial etiological agents of destructive periodontal diseases. Periodontol 2000 5, 78–111.[CrossRef]
    [Google Scholar]
  8. Ishihara, K., Miura, T., Kuramitsu, H. K. & Okuda, K. ( 1996; ). Characterization of the Treponema denticola prtP gene encoding a prolyl-phenyalanine-specific protease (dentilisin). Infect Immun 64, 5178–5186.
    [Google Scholar]
  9. Ishihara, K., Kuramitsu, H. K., Miura, T. & Okuda, K. ( 1998; ). Dentilisin activity affects the organization of the outer sheath of Treponema denticola. J Bacteriol 180, 3837–3844.
    [Google Scholar]
  10. Izard, J., Samsonoff, W. A. & Limberger, R. J. ( 2001; ). Cytoplasmic filament-deficient mutant of Treponema denticola has pleiotropic defects. J Bacteriol 183, 1078–1084.[CrossRef]
    [Google Scholar]
  11. Kataoka, M., Li, H., Arakawa, S. & Kuramitsu, H. ( 1997; ). Characterization of a methyl-accepting chemotaxis protein gene, dmcA, from the oral spirochete Treponema denticola. Infect Immun 65, 4011–4016.
    [Google Scholar]
  12. Kigure, T., Saito, A., Seida, K., Yamada, S., Ishihara, K. & Okuda, K. ( 1995; ). Distribution of Porphyromonas gingivalis and Treponema denticola in human subgingival plaque at different periodontal pocket depths examined by immunochemical methods. J Periodontol Res 30, 332–341.[CrossRef]
    [Google Scholar]
  13. Kimsey, R. B. & Spielman, A. ( 1990; ). Motility of Lyme disease spirochetes in fluids as viscous as the extracellular matrix. J Infect Dis 162, 1205–1208.[CrossRef]
    [Google Scholar]
  14. Klitorinos, A., Noble, P., Siboo, R. & Chan, E. C. ( 1993; ). Viscosity-dependent locomotion of oral spirochetes. Oral Microbiol Immunol 8, 242–244.[CrossRef]
    [Google Scholar]
  15. Kolenbrander, P. E. ( 1998; ). Intergeneric coaggregation among human oral bacteria and ecology of dental plaque. Annu Rev Microbiol 42, 627–656.
    [Google Scholar]
  16. Kolenbrander, P. E., Roxannan, A., Blehert, D. S., Egland, P. G., Foster, J. S. & Palmer, R. J., Jr ( 2002; ). Communication among oral bacteria. Microbiol Mol Biol Rev 66, 486–505.[CrossRef]
    [Google Scholar]
  17. Leung, W. K., Haapasalo, M., Uitto, V.-J., Hannam, P. M. & McBride, B. C. ( 1996; ). The surface proteinase of Treponema denticola may mediate attachment of the bacteria to epithelial cells. Anaerobe 2, 39–46.[CrossRef]
    [Google Scholar]
  18. Li, H., Ruby, J., Charon, N. & Kuramitsu, H. ( 1996; ). Gene inactivation in the oral spirochete Treponema denticola: construction of an flgE mutant. J Bacteriol 178, 3664–3667.
    [Google Scholar]
  19. Limberger, R. J., Slivienski, L. L., Izard, J. & Samsonoff, W. A. ( 1999; ). Insertional inactivation of Treponema denticola tap1 results in a nonmotile mutant with elongated flagellar hooks. J Bacteriol 181, 3743–3750.
    [Google Scholar]
  20. Loesche, W. J. ( 1988; ). The role of spirochetes in periodontal disease. Adv Dent Res 275–283.
    [Google Scholar]
  21. Loesche, W. J. ( 1993; ). Bacterial mediators in periodontal disease. Clin Infect Dis 16 (suppl. 44), S203–S210.
    [Google Scholar]
  22. O'Toole, G. A., Kaplan, H. B. & Kolter, R. ( 2000; ). Biofilm formation as microbial development. Annu Rev Microbiol 54, 49–79.[CrossRef]
    [Google Scholar]
  23. Pratt, L. A. & Kolter, P. ( 1998; ). Genetic analysis of Escherichia coli biofilm formation: roles of flagella, motility, chemotaxis and type I pili. Mol Microbiol 30, 285–293.[CrossRef]
    [Google Scholar]
  24. Saglie, R., Newman, M. G., Carranza, F. A. & Pattison, G. L. ( 1982; ). Bacterial invasion of gingiva in advanced periodontitis in humans. J Periodontol 53, 217–222.[CrossRef]
    [Google Scholar]
  25. Simonson, L. G., Goodman, C. H., Bial, J. J. & Morton, H. E. ( 1988; ). Quantitative relationship of Treponema denticola to severity of periodontal disease. Infect Immun 56, 726–728.
    [Google Scholar]
  26. Slots, J. & Genco, R. J. ( 1984; ). Black-pigmented Bacteroides species, Capnocytophaga species, and Actinobacillus actinomycetemcomitans in human periodontal disease: virulence factors in colonization, survival, and tissue destruction. J Dent Res 63, 412–421.[CrossRef]
    [Google Scholar]
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