1887

Abstract

is a major aetiological organism implicated in periodontal disease. The interaction of with other oral bacteria, in particular , in biofilm formation is thought to be an important step in the onset of periodontal disease. The interaction between and has been examined using a panel of mutants and their effects on mixed biofilm formation tested in a static biofilm model. ATCC 35405 did not form detectable biofilms on various inert surfaces. However, the spirochaete was demonstrated to form a biofilm with preattached 381. , which lacks the cytoplasmic filament, was unable to produce a mixed biofilm with . A mutant which lacks the flagella hook protein and is therefore non-motile displayed a reduced, but readily detectable, ability to form a mixed biofilm as did the mutant which does not possess the major outer sheath protein (Msp). The mutant was only moderately defective in forming mixed biofilms with . However, the methyl-accepting chemotaxis protein (DmcA) did not appear to play a major role in mixed biofilm formation. In contrast, lacking the PrtP protein for prolyl-phenylalanine-specific protease, showed an increased ability to form mixed biofilms and a prolonged viability in the biofilm.

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2004-07-01
2020-07-13
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