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Abstract

This paper focuses on global gene regulation by FlhD/FlhC in enteric bacteria. Even though FlhD/FlhC can complement an mutant for motility, it is not known if the FlhD/FlhC complex has an effect on metabolism similar to . To study metabolic gene regulation, a partial 8081c microarray was constructed and the expression patterns of wild-type cells were compared to an mutant strain at 25 and 37 °C. The overlap between the and FlhD/FlhC regulated genes was 25 %. Genes that were regulated at least fivefold by FlhD/FlhC in are genes encoding urocanate hydratase (), imidazolone propionase (), carbamoylphosphate synthetase () and aspartate carbamoyltransferase (). These enzymes are part of a pathway that is involved in the degradation of -histidine to -glutamate and eventually leads into purine/pyrimidine biosynthesis via carbamoylphosphate and carbamoylaspartate. A number of other genes were regulated at a lower rate. In two additional experiments, the expression of wild-type cells grown at 4 or 25 °C was compared to the same strain grown at 37 °C. The expression of the flagella master operon was not affected by temperature, whereas the flagella-specific sigma factor was highly expressed at 25 °C and reduced at 4 and 37 °C. Several other flagella genes, all of which are under the control of FliA, exhibited a similar temperature profile. These data are consistent with the hypothesis that temperature regulation of flagella genes might be mediated by the flagella-specific sigma factor FliA and not the flagella master regulator FlhD/FlhC.

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2004-07-01
2021-08-05
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