1887

Abstract

This study is focused on the involvement of the unusual nucleotide (p)ppGpp during the morphological and physiological differentiation of . In particular, the functional and structural elements of two genes encoding the proteins RelA and Rsh were identified. The gene encodes an 843 aa protein (RelA), while the gene encodes a 738 aa protein (Rsh). The and genes were disrupted by the insertion of a hygromycin resistance gene and an apramycin resistance gene, respectively. The synthesis of ppGpp in the gene-disrupted mutant was completely eliminated under conditions of starvation for amino acids, whereas synthesis persisted, but was greatly reduced in the gene-disrupted mutant. The gene-disrupted mutant had a bald appearance on agar plate cultures and retarded growth in submerged culture, while the -disrupted mutant was unchanged in growth characteristics relative to the wild-type culture. The production of both clavulanic acid and cephamycin C were completely abolished in the -disrupted mutant. Thus, it is concluded that the gene rather than is essential for morphological and physiological differentiation in and that RelA primarily governs the stringent response of to starvation for amino acids.

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2004-05-01
2019-11-12
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