1887

Abstract

Fifty strains belonging to , , and the recently described , were analysed using phenotypic and genomic techniques with the aim of analysing the usefulness of the different techniques for the identification of -related species. The species and were phenotypically indistinguishable by more than 100 phenotypic features. Thirty-nine experimental strains were phenotypically identified as , but FAFLP, REP-PCR, IGS-PCR and DNA–DNA hybridization proved that they in fact belong to the species . Similar groupings were found among all fingerprinting methodologies (except IGS-PCR). Thirty-two experimental strains clustered with the type and one reference strain; seven strains clustered with the type and three reference strains; and the type and four reference strains of grouped together. The correlations between DNA–DNA hybridization and the genomic fingerprinting by FAFLP and (GTG)-PCR were found to be above 0·68 and statistically significant, suggesting the value of the latter techniques for the reliable identification of -related species. The results presented indicate that strains phenotypically identified as are in fact ; these findings position as an important species involved in diseases of reared aquatic organisms.

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2004-06-01
2019-11-16
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