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Volume 150, Issue 3

RppA, a transducer homologue, and MmrA, a multidrug transporter homologue, are involved in the biogenesis and/or assembly of polysaccharide in Free

Abstract

cells move by gliding, and form multicellular fruiting bodies under conditions of starvation. The authors cloned a gene, designated (for receptor for polysaccharide production), which encodes a methyl-accepting protein homologous to the chemotaxis transducers in eubacteria. The gene was co-transcribed with , a gene homologous to various multidrug transporter genes. The or single mutants showed almost identical phenotypes to the wild-type strain; however, the double mutant exhibited reduced colony expansion, cell–cell agglutination and cellular reversal frequency. The double-mutant cells also showed less binding to Congo red, which mainly binds to fibril polysaccharide, than wild-type cells. Analysis of total polysaccharide in stationary-phase cells demonstrated that in the double mutant, polysaccharide levels were decreased by about 30 % as compared with the wild-type strain. These results indicated that RppA and MmrA play a role in the biogenesis and/or assembly of polysaccharide, and the phenotypes of the double mutant may be due to the reduction in fibril polysaccharide.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): A motility, adventurous motility , MCP, methyl-accepting chemotaxis protein , PE, phosphatidylethanolamine and S motility, social motility
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2004-03-01
2024-04-25
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RppA, a transducer homologue, and MmrA, a multidrug transporter homologue, are involved in the biogenesis and/or assembly of polysaccharide in Myxococcus xanthus
Microbiology 150, 631 (2004); https://doi.org/10.1099/mic.0.26786-0
/content/journal/micro/10.1099/mic.0.26786-0
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