1887

Abstract

sp. PCC 7120 was mutagenized by transposon Tn-1087b, generating a mutant whose heterocysts lack the envelope polysaccharide layer. The transposon was located between nucleotides 342 and 343 of , a 918 bp gene encoding a histidine kinase for a two-component regulatory system. Complementation of the mutant with a DNA fragment containing and targeted inactivation of the gene confirmed that is involved in heterocyst development. RT-PCR showed that was constitutively expressed in the presence or absence of a combined-nitrogen source. and , the two genes turned on during wild-type heterocyst development, were no longer activated in an -null mutant. The two-component signal transduction system involving may control the formation of the envelope polysaccharide layer and certain late events essential to the function of heterocysts.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.26747-0
2004-02-01
2019-10-22
Loading full text...

Full text loading...

/deliver/fulltext/micro/150/2/mic1500447.html?itemId=/content/journal/micro/10.1099/mic.0.26747-0&mimeType=html&fmt=ahah

References

  1. Albight, L. M., Huala, E. & Ausubel, F. M. ( 1989; ). Prokaryotic signal transduction mediated by sensor and regulator protein pairs. Annu Rev Genet 23, 311–336.[CrossRef]
    [Google Scholar]
  2. Bauer, C. C., Ramaswamy, K. S., Endley, S., Scappino, L. A., Golden, J. W. & Haselkorn, R. ( 1997; ). Suppression of heterocyst differentiation in Anabaena PCC 7120 by a cosmid carrying wild-type genes encoding enzymes for fatty acid synthesis. FEMS Microbiol Lett 151, 23–30.[CrossRef]
    [Google Scholar]
  3. Black, T. A. & Wolk, C. P. ( 1993; ). Spatial expression and autoregulation of hetR, a gene involved in the control of heterocyst development in Anabaena. Mol Microbiol 9, 77–84.[CrossRef]
    [Google Scholar]
  4. Black, T. A. & Wolk, C. P. ( 1994; ). Analysis of a het mutation in Anabaena sp. strain PCC 7120 implicates a secondary metabolite in the regulation of heterocyst spacing. J Bacteriol 176, 2282–2292.
    [Google Scholar]
  5. Black, K., Buikema, W. J. & Haselkorn, R. ( 1995; ). The hglK gene is required for localization of heterocyst-specific glycolipids in the cyanobacterium Anabaena sp. strain PCC 7120. J Bacteriol 177, 6440–6448.
    [Google Scholar]
  6. Cai, Y. & Wolk, C. P. ( 1997; ). Anabaena sp. strain PCC 7120 responds to nitrogen deprivation with a cascade-like sequence of transcriptional activations. J Bacteriol 179, 267–271.
    [Google Scholar]
  7. Callahan, S. M. & Buikema, W. J. ( 2001; ). The role of HetN in maintenance of the heterocyst pattern in Anabaena sp. PCC 7120. Mol Microbiol 40, 941–950.[CrossRef]
    [Google Scholar]
  8. Campbell, E. L., Cohen, M. F. & Meeks, J. C. ( 1997; ). A polyketide-synthase-like gene is involved in the synthesis of heterocyst glycolipids in Nostoc punctiforme strain ATCC 29133. Arch Microbiol 167, 251–258.[CrossRef]
    [Google Scholar]
  9. Elhai, J. & Wolk, C. P. ( 1988; ). Conjugal transfer of DNA to cyanobacteria. Methods Enzymol 167, 747–754.
    [Google Scholar]
  10. Ernst, A., Black, T., Cai, Y., Panoff, J. M., Tiwari, D. N. & Wolk, C. P. ( 1992; ). Synthesis of nitrogenase in mutants of the cyanobacterium Anabaena sp. strain PCC 7120 affected in heterocyst development or metabolism. J Bacteriol 174, 6025–6032.
    [Google Scholar]
  11. Fiedler, G., Arnold, M., Hannus, S. & Maldener, I. ( 1998; ). The DevBCA exporter is essential for envelope formation in heterocysts of the cyanobacterium Anabaena sp. strain PCC 7120. Mol Microbiol 27, 1193–1202.[CrossRef]
    [Google Scholar]
  12. Hebbar, P. B. & Curtis, S. E. ( 2000; ). Characterization of devH, a gene encoding a putative DNA binding protein required for heterocyst function in Anabaena sp. strain PCC 7120. J Bacteriol 182, 3572–3581.[CrossRef]
    [Google Scholar]
  13. Holland, D. & Wolk, C. P. ( 1990; ). Identification and characterization of hetA, a gene that acts early in the process of morphological differentiation of heterocysts. J Bacteriol 172, 3131–3137.
    [Google Scholar]
  14. Jones, K. M., Buikema, W. J. & Haselkorn, R. ( 2003; ). Heterocyst-specific expression of patB, a gene required for nitrogen fixation in Anabaena sp. strain PCC 7120. J Bacteriol 185, 2306–2314.[CrossRef]
    [Google Scholar]
  15. Kaneko, T., Nakamura, Y., Wolk, C. P. & 19 other authors ( 2001; ). Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120. DNA Res 8, 205–213.[CrossRef]
    [Google Scholar]
  16. Liang, J., Scappino, L. & Haselkorn, R. ( 1992; ). The patA gene product, which contains a region similar to CheY of Escherichia coli, controls heterocyst pattern formation in the cyanobacterium Anabaena 7120. Proc Natl Acad Sci U S A 89, 5655–5659.[CrossRef]
    [Google Scholar]
  17. Maldener, I., Fiedler, G., Ernst, A., Fernandez-Pinas, F. & Wolk, C. P. ( 1994; ). Characterization of devA, a gene required for the maturation of proheterocysts in the cyanobacterium Anabaena sp. strain PCC 7120. J Bacteriol 176, 7543–7549.
    [Google Scholar]
  18. Murry, M. A. & Wolk, C. P. ( 1989; ). Evidence that the barrier to the penetration of oxygen into heterocysts depends upon two layers of the cell envelope. Arch Microbiol 151, 469–474.[CrossRef]
    [Google Scholar]
  19. Song, L., Liu, Y., Gan, X., Zhu, Y. & Yu, M. ( 1999; ). FACHB-collection: general introduction and the strain list. Acta Hydrobiol Sin 23 (Suppl.), 537–546.
    [Google Scholar]
  20. Stanier, R. Y., Kunisawa, R., Mandel, M. & Cohen-Bazire, G. ( 1971; ). Purification and properties of unicellular blue-green algae (order Chroococcales). Bacteriol Rev 35, 171–205.
    [Google Scholar]
  21. Stock, J. B., Surette, M. G., Levit, M. & Park, P. ( 1995; ). Two-component signal transduction systems: structure-function relationships and mechanisms of catalysis. In Two Component Signal Transduction, pp. 25–51. Edited by J. A. Hoch & T. J. Silhavy. Washington, DC: American Society for Microbiology.
  22. Stock, A. M., Robinson, V. L. & Goudreau, P. N. ( 2000; ). Two-component signal transduction. Annu Rev Biochem 69, 183–215.[CrossRef]
    [Google Scholar]
  23. Walsby, A. E. ( 1985; ). The permeability of heterocysts to the gases nitrogen and oxygen. Proc R Soc Lond B 226, 345–366.[CrossRef]
    [Google Scholar]
  24. Wei, T.-F., Ramasubramanian, T. S. & Golden, J. W. ( 1994; ). Anabaena sp. strain PCC 7120 ntcA gene required for growth on nitrate and heterocyst development. J Bacteriol 176, 4473–4482.
    [Google Scholar]
  25. Wolk, C. P. ( 2000; ). Heterocyst formation in Anabaena. In Prokaryotic Development, pp. 83–104. Edited by Y. V. Brun & L. J. Shimkets. Washington, DC: American Society for Microbiology.
  26. Wolk, C. P., Cai, Y., Cardemil, L. & 6 other authors ( 1988; ). Isolation and complementation of mutation of Anabaena sp. strain PCC7120 unable to grow aerobically on dinitrogen. J Bacteriol 170, 1239–1244.
    [Google Scholar]
  27. Wolk, C. P., Elhai, J., Kuritz, T. & Holland, D. ( 1993; ). Amplified expression of a transcriptional pattern formed during development of Anabaena. Mol Microbiol 7, 441–445.[CrossRef]
    [Google Scholar]
  28. Xu, X. & Wolk, C. P. ( 2001; ). Role for hetC in the transition to a nondividing state during heterocyst differentiation in Anabaena sp. J Bacteriol 183, 393–396.[CrossRef]
    [Google Scholar]
  29. Xu, X., Kong, R., de Bruijn, F. J., He, S. Y., Murry, M. A., Newman, T. & Wolk, C. P. ( 2002; ). DNA sequence and genetic characterization of plasmid pFQ11 from Frankia alni strain CpI1. FEMS Microbiol Lett 207, 103–107.[CrossRef]
    [Google Scholar]
  30. Zhou, R. & Wolk, C. P. ( 2003; ). A two-component system mediates developmental regulation of biosynthesis of a heterocyst polysaccharide. J Biol Chem 278, 19939–19946.[CrossRef]
    [Google Scholar]
  31. Zhu, J., Kong, R. & Wolk, C. P. ( 1998; ). Regulation of hepA of Anabaena sp. strain PCC 7120 by elements 5′ from the gene and by hepK. J Bacteriol 180, 4233–4242.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.26747-0
Loading
/content/journal/micro/10.1099/mic.0.26747-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error