1887

Abstract

sp. PCC 7120 was mutagenized by transposon Tn-1087b, generating a mutant whose heterocysts lack the envelope polysaccharide layer. The transposon was located between nucleotides 342 and 343 of , a 918 bp gene encoding a histidine kinase for a two-component regulatory system. Complementation of the mutant with a DNA fragment containing and targeted inactivation of the gene confirmed that is involved in heterocyst development. RT-PCR showed that was constitutively expressed in the presence or absence of a combined-nitrogen source. and , the two genes turned on during wild-type heterocyst development, were no longer activated in an -null mutant. The two-component signal transduction system involving may control the formation of the envelope polysaccharide layer and certain late events essential to the function of heterocysts.

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2004-02-01
2020-09-21
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