1887

Abstract

Lysylphosphatidylglycerol (LPG) is a basic phospholipid in which -lysine from lysyl-tRNA is transferred to phosphatidylglycerol (PG). This study examined whether the S gene encodes LPG synthetase. A crude membrane fraction prepared from wild-type cells had LPG synthetase activity that depended on PG and lysyl-tRNA, whereas the membrane fraction from an deletion mutant did not. When MprF protein was trans-expressed in wild-type cells, LPG synthesis was induced, whereas it was not observed in mutant cells in which the amount of PG is significantly reduced. In addition, LPG synthetase activity and a 93 kDa protein whose molecular size corresponded to that of MprF protein were co-induced in the crude membrane fraction prepared from cells expressing MprF protein. The values of the LPG synthetase activity for PG and for lysyl-tRNA were 56 μM and 6·9 μM, respectively, consistent with those of membranes. These results suggest that the MprF protein is LPG synthetase.

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2004-01-01
2019-10-20
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