1887

Abstract

The sporulation-specific SpoVIF (YjcC) protein of is essential for the development of heat-resistant spores. The GerE protein, the smallest member of the LuxR-FixJ family, contains a helix–turn–helix (HTH) motif and is involved in the expression of various sporulation-specific genes. In this study, the gene expression and protein composition of sporulating -negative cells were analysed. CgeA, CotG and CotS, which are GerE-dependent coat proteins, were not expressed in the -negative cells. Northern blotting showed that SpoVIF regulated the transcription of , and in a manner similar to that of GerE. In -negative cells, mRNA was transcribed normally, but immunoblot analysis using anti-GerE antiserum showed that the quantity of GerE protein was considerably less than that in wild-type controls. Using GFP (green fluorescent protein) fusion proteins, the localization of SpoVIF and GerE was observed by fluorescence microscopy. SpoVIF-GFP was detectable in the mother cell compartment, as was GerE-GFP. These results suggest that SpoVIF directly or indirectly controls the function of the GerE protein, and that SpoVIF is required for gene regulation during the latter stages of sporulation.

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2004-01-01
2020-04-07
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