1887

Abstract

The roles of two adjacent genes in the chromosome with functions in starvation survival and the response to stressful conditions have been characterized. One of these, , encoding a hypoxanthine–guanine phosphoribosyltransferase homologue, was initially identified in a transposon mutagenesis screen. Mutation of affects starvation survival in amino-acid-limiting conditions and the ability of to grow in high-salt concentrations. Downstream of is , which encodes a membrane-bound, ATP- and Zn-dependent ‘AAA’-type protease. Mutation of in leads to pleiotropic defects including slower growth, sensitivity to salt, acid, methyl viologen and potassium tellurite stresses, and reduced survival in amino-acid- or phosphate-limiting conditions. Both and gene fusions are expressed maximally in the post-exponential phase of growth. Although secretion of exoproteins is not affected, an mutant is attenuated in a murine skin lesion model of pathogenicity.

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2004-02-01
2020-08-08
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