1887

Abstract

Coronamycin is a complex of novel peptide antibiotics with activity against pythiaceous fungi and the human fungal pathogen . It is also active against the malarial parasite, , with an IC of 9·0 ng ml. Coronamycin is produced by a verticillate sp. isolated as an endophyte from an epiphytic vine, sp., found in the Manu region of the upper Amazon of Peru. Bioassay-guided fractionation of the fermentation broths of this endophyte on silica gel and HPLC chromatography yielded two principal, inseparable, peptides with masses of 1217·9 and 1203·8 Da. Three other minor, but related components, are also present in the preparation. Amino acid analysis of coronamycin revealed residues of component 1, component 2, methionine, tyrosine and leucine at a ratio of 2 : 2 : 1 : 1 : 3. Other compounds with antifungal activities are also produced by this endophytic streptomycete.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.26645-0
2004-04-01
2020-04-01
Loading full text...

Full text loading...

/deliver/fulltext/micro/150/4/mic1500785.html?itemId=/content/journal/micro/10.1099/mic.0.26645-0&mimeType=html&fmt=ahah

References

  1. Arai T.. 1976; Actinomycetes: the Boundary Microorganisms Singapore: Toppan;
  2. Bacon C. W., White J. F.. 2000; Microbial Endophytes New York: Marcel Dekker;
    [Google Scholar]
  3. Ballio A., Bossa F., DiGiogio P., Ferranti P., Paci M., Pucci P., Scaloni A., Segre A., Strobel G. A.. 1994; Structure of the pseudomycins, new lipodepsipeptides produced by Pseudomonas syringae MSU 16H. FEBS Lett355:96–100[CrossRef]
    [Google Scholar]
  4. Castillo U., Strobel G. A., Ford E. J..9 other authors 2002; Munumbicins, wide spectrum antibiotics produced by Streptomyces munumbi, endophytic on Kennedia nigriscans. Microbiology148:2675–2685
    [Google Scholar]
  5. Castillo U., Harper J. K., Strobel G. A..16 other authors 2003; Kakadumycins, novel antibiotics from Streptomyces sp. NRRL 30566, an endophyte of Grevillea pteridifolia. FEMS Lett224:183–190[CrossRef]
    [Google Scholar]
  6. Cohen S., Michaud D.. 1993; Compositional protein analysis using 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate, a novel derivatization reagent. Tech Protein Chem4:289–298
    [Google Scholar]
  7. Demain A.. 1981; Industrial microbiology. Science214:987–994[CrossRef]
    [Google Scholar]
  8. Espinel-Ingroff A., Pfaller M., Messer S. A., Knapp C. C., Killian S., Norris H. A., Ghannoum M. A.. 1999; Multicenter comparison of the sensititre YeastOne colorimetric antifungal panel with the National Committee for Clinical Laboratory Standards M27-A reference methods for testing of clinical isolates of common and emerging Candida spp., Cryptococcus spp., and other yeasts and yeast-like organisms. J Clin Microbiol37:591–595
    [Google Scholar]
  9. Goodfellow M., Williams S. T., Mordarski M.. 1988; Actinomycetes in Biotechnology London: Academic Press;
  10. Kunoh H.. 2002; Endophytic actinomycetes: attractive biocontrol agents. J Gen Plant Pathol68:249–252[CrossRef]
    [Google Scholar]
  11. Lane D. L.. 1991; 16S/23S rDNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp.115–176 Edited by Stackebrandt E., Goodfellow M.. Chichester: Wiley;
    [Google Scholar]
  12. Locci R., Schofield G. M.. 1989; Genus Streptoverticillium Baldacci 1958, 15, emend. mut. char. Baldacci, Farinia and Locci 1966, 168AL. In Bergey's Manuel of Systematic Bacteriology vol 4 pp.2492–2501 Edited by Williams S. T., Sharpe M. E., Holt J. E.. Baltimore, MD: Williams & Wilkins;
  13. Miller C. M., Miller R. V., Garton-Kinney D., Redgrave B., Sears J., Condron M., Teplow D., Strobel G. A.. 1998; Ecomycins, unique antimycotics from Pseudomonas viridiflava. J Appl Microbiol4:937–944
    [Google Scholar]
  14. Miyadoh S.. 1997; Atlas of Actinomyctes Japan: Asakura;
    [Google Scholar]
  15. NCCLS 1997; Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved Standard NCCLS document M27-A Wayne, PA: NCCLS;1-56238-328-0
  16. NIH 2001; : NIAID Global Health Research Plan for HIV/AIDS, Malaria and Tuberculosis Bethesda, MD: US Department of Health and Human Services;
    [Google Scholar]
  17. Pouchert C. J., Campbell J. R.. 1974; The Aldrich Library of NMR Spectra Milwaukee, WI: Aldrich;
  18. Silverstein R. M., Bassler G. C., Morrill T. C.. 1991; Spectrometric Identification of Organic Compounds New York: Wiley;
  19. Stinson M., Ezra D., Sears J., Hess W. M., Strobel G.. 2003; An endophytic Gliocladium sp. of Eucryphia cordifolia producing selective volatile antimicrobial compounds. Plant Sci165:913–922[CrossRef]
    [Google Scholar]
  20. Strobel G. A.. 2002; Rainforest endophytes and bioactive products. Crit Rev Biotechnol22:315–333[CrossRef]
    [Google Scholar]
  21. Strobel G.. 2003; Endophytes as sources of bioactive products. Microbes Infect5:535–544[CrossRef]
    [Google Scholar]
  22. Strobel G. A., Miller R. V., Miller C., Condron M., Teplow D. B., Hess W. M.. 1999; Cryptocandin, a potent antimycotic from the endophytic fungus Cryptosporiopsis cf.quercina. Microbiology145:1919–1926[CrossRef]
    [Google Scholar]
  23. Trager W., Jensen J. B.. 1976; Human malaria parasites in continuous culture. Science193:673–675[CrossRef]
    [Google Scholar]
  24. Trager W., Jensen J. B.. 1978; Cultivation of malarial parasites. Nature273:621–622[CrossRef]
    [Google Scholar]
  25. Waring M. J.. 1979; Echinomycin, triosin and related antibiotics. In Antibiotics, Mechanism of Action of Antieukaryotic and Antiviral Compounds vol. 5, part 2 pp.173–194 Edited by Hahn F. E.. Heidelberg: Springer;
    [Google Scholar]
  26. Witt D., Stackebrandt E.. 1990; Unification of the genera Streptoverticillium and Streptomyces and amendation of the Streptomyces Waksman and Henrici 1943, 339AL. Syst Appl Microbiol13:361–371[CrossRef]
    [Google Scholar]
  27. Zhang Y. Z., Sun X., Zeckner D., Sachs B., Current W., Chen S. H.. 2001; 8-Amido-bearing pseudomycin B (PSB) analogue: novel antifungal agents. Bioorg Med Chem Lett11:123–126[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.26645-0
Loading
/content/journal/micro/10.1099/mic.0.26645-0
Loading

Data & Media loading...

Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error