1887

Abstract

The hexanucleotide CCAGCA was found repeated 15 times in tandem on the 5′ side of the virginiamycin acetyl transferase gene of strain Y56. The corresponding region was analysed by PCR from 54 clinical strains belonging to the same biotype and serotype, and others from this laboratory collection belonging to different biotypes and serotypes. Each strain produced a single amplification product whose size was variable among strains, revealing that the locus was polymorphic. Nucleotide sequence determination of selected PCR products showed that the polymorphism was due to the precise expansion or reduction in the number of hexanucleotide repeats. Analysis of this locus in a few strains showing the same PFGE pattern showed that it was also polymorphic. These results suggest that this method could be valuable to increase the discriminatory power of current typing schemes.

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2004-01-01
2024-03-29
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References

  1. Adair D. M., Worsham P. L., Hill K. K., Klevytska A. M., Jackson P. J., Friedlander A. M., Keim P. 2000; Diversity in a variable-number tandem repeat from Yersinia pestis. J Clin Microbiol 38:1516–1519
    [Google Scholar]
  2. Bayliss C. D., Field D., Moxon E. R. 2001; The simple sequence contingency loci of Haemophilus influenzae and Neisseria meningitidis. J Clin Invest 107:657–662 [CrossRef]
    [Google Scholar]
  3. Bricker B. J., Ewalt D. R., Halling S. M. 2003; Brucella ‘HOOF-Prints’: strain typing by multi-locus analysis of variable number tandem repeats (VNTRs. BMC Microbiol 3:15 [CrossRef]
    [Google Scholar]
  4. Deng W., Burland V., Plunkett G. 3rd18 other authors 2002; Genome sequence of Yersinia pestis. KIM. J Bacteriol 184:4601–4611 [CrossRef]
    [Google Scholar]
  5. Frothingham R., Meeker-O'Connell W. A. 1998; Genetic diversity in the Mycobacterium tuberculosis complex based on variable numbers of tandem DNA repeats. Microbiology 144:1189–1196 [CrossRef]
    [Google Scholar]
  6. Hallanvuo S., Skurnik M., Asplund K., Siitonen A. 2002; Detection of a novel repeated sequence useful for epidemiological typing of pathogenic Yersinia enterocolitica. Int J Med Microbiol 292:215–225 [CrossRef]
    [Google Scholar]
  7. Iteman I., Guiyoule A., Carniel E. 1996; Comparison of three molecular methods for typing and subtyping pathogenic Yersinia enterocolitica strains. . J Med Microbiol 45:48–56 [CrossRef]
    [Google Scholar]
  8. Kashi Y., King D., Soller M. 1997; Simple sequence repeats as a source of quantitative genetic variation. Trends Genet 13:74–78 [CrossRef]
    [Google Scholar]
  9. Keim P., Price L. B., Klevytska A. M., Smith K. L., Schupp J. M., Okinaka R., Jackson P. J., Hugh-Jones M. E. 2000; Multiple-locus variable-number tandem repeat analysis reveals genetic relationships within Bacillus anthracis. J Bacteriol 182:2928–2936 [CrossRef]
    [Google Scholar]
  10. Leyva J., Olmo C., García-Jalón I., Irigoyen A., Oteiza C., Dorronsoro I., Diaz R. 1995; Coagglutination with antisera to MyfA and PsaA to distinguish Yersinia enterocolitica from Yersinia pseudotuberculosis pathogenic isolates. . Contrib Microbiol Immunol 13:158–164
    [Google Scholar]
  11. Madoff L. C., Michel J. L., Gong E. W., Kling D. E., Kasper D. L. 1996; Group B streptococci escape host immunity by deletion of tandem repeat elements of the alpha C protein. Proc Natl Acad Sci U S A 93:4131–4136 [CrossRef]
    [Google Scholar]
  12. Parkhill J., Wren B. W., Thomson N. R. & 26 other authors; 2001; Genome sequence of Yersinia pestis, the causative agent of plague. Nature 413:523–527 [CrossRef]
    [Google Scholar]
  13. Ritz D., Lim J., Reynolds C. M., Poole L. B., Beckwith J. 2001; Conversion of a peroxiredoxin into a disulfide reductase by a triplet repeat expansion. Science 294:158–160 [CrossRef]
    [Google Scholar]
  14. Saken E., Roggenkamp A., Aleksic S., Heesemann J. 1994; Characterisation of pathogenic Yersinia enterocolitica serogroups by pulsed-field gel electrophoresis of genomic NotI restriction fragments. J Med Microbiol 41:329–338 [CrossRef]
    [Google Scholar]
  15. Saunders N. J., Jeffries A. C., Peden J. F., Hood D. W., Tettelin H., Rappuoli R., Moxon E. R. 2000; Repeat-associated phase variable genes in the complete genome sequence of Neisseria meningitidis strain MC58. . Mol Microbiol 37:207–215 [CrossRef]
    [Google Scholar]
  16. Seoane A., García Lobo J. M. 1991; Cloning of chromosomal β-lactamase genes from Yersinia enterocolitica. J Gen Microbiol 137:141–146 [CrossRef]
    [Google Scholar]
  17. Seoane A., García Lobo J. M. 2000; Identification of a streptogramin A acetyltransferase gene in the chromosome of Yersinia enterocolitica. Antimicrob Agents Chemother 44:905–909 [CrossRef]
    [Google Scholar]
  18. Smith G. A., Theriot J. A., Portnoy D. A. 1996; The tandem repeat domain in the Listeria monocytogenes ActA protein controls the rate of actin-based motility, the percentage of moving bacteria, and the localization of vasodilator-stimulated phosphoprotein and profilin. J Cell Biol 135:647–660 [CrossRef]
    [Google Scholar]
  19. van Belkum A. 1999; The role of short sequence repeats in epidemiologic typing. Curr Opin Microbiol 2:306–311 [CrossRef]
    [Google Scholar]
  20. van Belkum A., Scherer S., van Leeuwen W., Willemse D., van Alphen L., Verbrugh H. 1997; Variable number of tandem repeats in clinical strains of Haemophilus influenzae. Infect Immun 65:5017–5027
    [Google Scholar]
  21. Verkerk A. J., Pieretti M., Sutcliffe J. S.7 other authors 1991; Identification of a gene (FMR-1) containing a CGG repeat coincident with a breakpoint cluster region exhibiting length variation in fragile X syndrome. Cell 65:905–914 [CrossRef]
    [Google Scholar]
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