1887

Abstract

Examination of the nucleotide sequences of the 16S–23S intergenic transcribed spacer (ITS) region of and identified a putative transposase gene located only in the ITS of , which can be used as a genetic marker to distinguish these two species. The relative size of the PCR products of the ITS region allowed a clear distinction to be made between strains of and , both of which could be readily discriminated from the type strains of all the other recognized avian species. In addition, the putative transposase gene assigned in the ITS of was shown to include a sequence homologous to that of the P75 gene of . This is believed to be the first description of an insertion element in the rRNA operon region of a mycoplasma species.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.26629-0
2004-04-01
2020-08-11
Loading full text...

Full text loading...

/deliver/fulltext/micro/150/4/mic1501023.html?itemId=/content/journal/micro/10.1099/mic.0.26629-0&mimeType=html&fmt=ahah

References

  1. Adler H. E.. 1960; Mycoplasma, the cause of chronic respiratory disease. Ann N Y Acad Sci79:703–712
    [Google Scholar]
  2. Altschul S. F., Madden T. L., Schaffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J.. 1997; Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res25:3389–3402[CrossRef]
    [Google Scholar]
  3. Berg K. L., Squires C., Squires C. L.. 1989; Ribosomal RNA operon anti-termination function of leader and spacer region boxB-boxA sequences and their conservation in diverse microorganisms. J Mol Biol209:345–358[CrossRef]
    [Google Scholar]
  4. Bhugra B., Dybvig K.. 1993; Identification and characterization of IS1138, a transposable element from Mycoplasma pulmonis that belongs to the IS3 family. Mol Microbiol7:577–584[CrossRef]
    [Google Scholar]
  5. Bradbury J. M.. 1977; Rapid biochemical tests for characterization of the Mycoplasmatales. J Clin Microbiol5:531–534
    [Google Scholar]
  6. Bradbury J. M., Abdul-Wahab O. M. S., Yavari C. A., Dupiellet J.-P., Bové J. M.. 1993; Mycoplasma imitans sp. nov. is related to Mycoplasma gallisepticum and found in birds. Int J Syst Bacteriol43:721–728[CrossRef]
    [Google Scholar]
  7. Buntz B., Bradbury J. M., Vuillaume A., Rousselot-Paillet D.. 1986; Isolation of Mycoplasma gallisepticum from geese. Avian Pathol15:615–617[CrossRef]
    [Google Scholar]
  8. Calcutt M. J., Kim M. F., Karpas A. B., Mühlradt P. F., Wise K. S.. 1999; Differential posttranslational processing confers intraspecies variation of a major surface lipoprotein and a macrophage-activating lipopeptide of Mycoplasma fermentans. Infect Immun67:760–771
    [Google Scholar]
  9. Chiaruttini C., Milet M.. 1993; Gene organization, primary structure and RNA processing analysis of a ribosomal RNA operon in Lactobacillus lactis. J Mol Biol230:57–76[CrossRef]
    [Google Scholar]
  10. Dupiellet J.-P.. 1984; Mycoplasmes et Acholeplasmes des Palmipèdes à Foie Gras: Isolement, Caractérisation, Études du Rôle dans la Pathologie. Rapport de DEA de Pathologie, Université de Bordeaux II Villeneuve d'Ornon, France:
    [Google Scholar]
  11. Dupiellet J.-P.. 1988; Mycoplasmes de l'oie et du canard: contribution à l'étude sérologique et moléculaire de souches aparentées à Mycoplasma gallisepticum. PhD thesis Université de Bordeaux II; Villenave d'Ornon, France:
    [Google Scholar]
  12. Dupiellet J.-P., Vuillaume A., Rousselot D., Bové J. M., Bradbury J. M.. 1988; Serological and molecular studies on Mycoplasma gallisepticum strains. In Abstracts of the 7th Congress of the International Organization for Mycoplasmologyp– 150
    [Google Scholar]
  13. Dupiellet J.-P., Vuillaume A., Rousselot D., Bové J. M., Bradbury J. M.. 1990; Serological and molecular studies on Mycoplasma gallisepticum strains. Zentbl Bakteriol Suppl20:859–864
    [Google Scholar]
  14. Fox G. E., Wisotzkey J. D., Jurtshuk P. Jr. 1992; How close is close: 16S rRNA sequence identity may not be sufficient to guarantee species identity. Int J Syst Bacteriol42:166–170[CrossRef]
    [Google Scholar]
  15. Gürtler V.. 1999; The role of recombination and mutation in 16S-23S rDNA spacer rearrangements. Gene238:241–252[CrossRef]
    [Google Scholar]
  16. Gürtler V., Stanisich V. M.. 1996; New approaches to typing and identification of bacteria using the 16S-23S rDNA spacer region. Microbiology142:3–16[CrossRef]
    [Google Scholar]
  17. Harasawa R.. 1996; PCR: application of nested PCR to detection of Mycoplasmas. In Molecular and Diagnostic Procedures in Mycoplasmology vol 2 pp.75–79Edited by Razin S., Tully J. G.. New York: Academic Press;
    [Google Scholar]
  18. Harasawa R.. 1999; Genetic relationships among mycoplasmas based on the 16S-23S rRNA spacer sequence. Microbiol Immunol43:127–132[CrossRef]
    [Google Scholar]
  19. Harasawa R., Lefkowitz E. J., Glass J. I., Cassell G. H.. 1996; Phylogenetic analysis of the 16S-23S rRNA intergenic spacer region of the genus Ureaplasma. J Vet Med Sci8:191–195
    [Google Scholar]
  20. Lane D. J.. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp.115–148Edited by Stackebrandt E., Goodfellow M.. Chichester, UK: Wiley;
    [Google Scholar]
  21. Liiv A., Tenson T., Morgus T., Remme J.. 1998; Multiple functions of the transcribed spacers in ribosomal RNA operon. Biol Chem379:783–793
    [Google Scholar]
  22. Lim D., Maas W. K.. 1989; Reverse transcriptase in bacteria. Mol Microbiol3:1141–1144[CrossRef]
    [Google Scholar]
  23. Lou Y. K., Smith D. L., Robinson B. G., Morris B. J.. 1991; Renin gene expression in various tissues determined by single-step polymerase chain reaction. Clin Exp Pharmacol Physiol18:357–362[CrossRef]
    [Google Scholar]
  24. Myers T. W., Gelfand D. H.. 1991; Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase. Biochemistry30:7661–7666[CrossRef]
    [Google Scholar]
  25. Papazisi L., Gorton T. S., Kutish G..7 other authors 2003; The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain Rlow. Microbiology 149:2307–2316[CrossRef]
    [Google Scholar]
  26. Pilo P., Fleury B., Marenda M., Frey J., Vilei E. M.. 2003; Prevalence and distribution of the insertion element ISMag1 in Mycoplasma agalactiae. Vet Microbiol92:37–48[CrossRef]
    [Google Scholar]
  27. Razin S.. 1985; Molecular biology and genetics of mycoplasmas (Mollicutes. Microbiol Rev49:419–455
    [Google Scholar]
  28. Rosendal S., Black F. T.. 1972; Direct and indirect immunofluorescence of unfixed and fixed Mycoplasma colonies. Acta Pathol Microbiol Scand Sect B Microbiol Immunol80:615–622
    [Google Scholar]
  29. Sasaki Y., Ishikawa L., Yamashita A.. & 8 other authors. 2002; The complete genomic sequence of Mycoplasma penetrans, an intracellular bacterial pathogen in humans. Nucleic Acids Res30:5293–5300[CrossRef]
    [Google Scholar]
  30. Spencer D. L., Kurth K. T., Menon A. A., VanDyk T., Minion F. C.. 2002; Cloning and analysis of the gene for a major surface antigen of Mycoplasma gallisepticum. Avian Dis46:816–825[CrossRef]
    [Google Scholar]
  31. Swofford D. L., Olsen G. J.. 1990; Phylogenetic reconstruction. In Molecular Systematics pp.411–501Edited by Hillis D. M., Moritz C.. Sunderland, MA: Sinauer Associates;
    [Google Scholar]
  32. Thompson J. D., Gibson T. J., Plewniak F., Jaenmougin F., Higgins D. G.. 1997; The CLUSTAL X Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res25:4876–4882[CrossRef]
    [Google Scholar]
  33. Varmus H. E.. 1989; Reverse transcription in bacteria. Cell56:721–724[CrossRef]
    [Google Scholar]
  34. Vilei E. M., Nicolet J., Frey J.. 1999; IS1634, a novel insertion element creating long, variable-length direct repeats which is specific for Mycoplasma mycoides subsp. mycoides small-colony type. J Bacteriol181:1319–1323
    [Google Scholar]
  35. Yogev D., Levisohn S., Kleven S. H., Halachmi D., Razin S.. 1988a; Ribosomal RNA gene probes to detect intraspecies heterogeneity in Mycoplasma gallisepticum and M. synoviae. Avian Dis32:220–231[CrossRef]
    [Google Scholar]
  36. Yogev D., Sela S., Bercovier H., Razin S.. 1988b; Elongation factor (EF-Tu) gene probe detects polymorphism in Mycoplasma strains. FEMS Microbiol Lett50:145–149[CrossRef]
    [Google Scholar]
  37. Zander D. V.. 1961; Origin of the S6 strain Mycoplasma. Avian Dis5:154–156[CrossRef]
    [Google Scholar]
  38. Zheng J., McIntosh M. A.. 1995; Characterization of IS1221 from Mycoplasma hyorhinis: expression of its putative transposase in Escherichia coli incorporates a ribosomal frame shift mechanism. Mol Microbiol16:669–685[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.26629-0
Loading
/content/journal/micro/10.1099/mic.0.26629-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error