RT Journal Article SR Electronic(1) A1 Martínez, Marta A1 Brito, Belén A1 Imperial, Juan A1 Ruiz-Argüeso, TomásYR 2004 T1 Characterization of a new internal promoter (P3) for Rhizobium leguminosarum hydrogenase accessory genes hupGHIJ JF Microbiology, VO 150 IS 3 SP 665 OP 675 DO https://doi.org/10.1099/mic.0.26623-0 PB Microbiology Society, SN 1465-2080, AB Synthesis of the Rhizobium leguminosarum [NiFe] hydrogenase requires the participation of 16 accessory genes (hupCDEFGHIJKhypABFCDEX) besides the genes encoding the structural proteins (hupSL). Transcription of hupSL is controlled by a −24/−12-type promoter (P1), located upstream of hupS and regulated by NifA. In this work, a second −24/−12-type promoter (P3), located upstream of the hupG gene and transcribing hupGHIJ genes in R. leguminosarum pea (Pisum sativum L.) bacteroids, has been identified in the hup gene cluster. Promoter P3 was also active in R. leguminosarum free-living cells, as evidenced by genetic complementation of hydrogenase mutants. Both NifA and NtrC activated P3 expression in the heterologous host Klebsiella pneumoniae. Also, P3 activity was highly stimulated by K. pneumoniae NifA in Escherichia coli. This NifA activation of P3 expression only required the σ 54-binding site, and it was independent of any cis-acting element upstream of the σ 54 box, which suggests a direct interaction of free NifA with the RNA polymerase holoenzyme. P3-dependent hupGHIJ expression in pea nodules started in interzone II/III, spanned through nitrogen-fixing zone III, and was coincident with the NifA-dependent nifH expression pattern. However, P3 was dispensable for hupGHIJ transcription and hydrogenase activity in pea bacteroids due to transcription initiated at P1. This fact and the lack of an activator recruitment system suggest that P3 plays a secondary role in symbiotic hupGHIJ expression., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.26623-0