1887

Abstract

Ochratoxin A is an important nephrotoxic and nephrocarcinogenic mycotoxin, produced by as a polyketide-derived secondary metabolite. A portion of a putative polyketide synthase gene () involved in the biosynthesis of this mycotoxin was cloned by using a suppression subtractive hybridization PCR-based approach. The predicted amino acid sequence of the 1·4 kb clone shared 28–35 % identity to acyl transferase regions from fungal polyketide synthases found in the databases. Based on reverse transcription PCR studies, the gene is expressed only under ochratoxin A permissive conditions and only during the early stages of the mycotoxin synthesis. A mutant in which the gene has been interrupted cannot synthesize ochratoxin A. This report is the first of the cloning and characterization of a gene involved in ochratoxin A biosynthesis.

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2003-12-01
2019-10-23
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